Study On The Effect Of "Astragalus-Safflower" On Wound Healing After Trauma In SD Rats Based On PI3K-Akt-mTOR Signaling Pathwa

Objective: Based on the PI3K-Akt-mTOR signaling pathway,this study was conducted to explore the effects of "Astragalus-Safflower" on wound healing in SD rats with the common clinical evidence of Qi deficiency and blood stasis.Methods: SD rats were randomly divided into the five groups:the blank control group,the model control group,the low,medium and high doses of astragalus-safflower solution group.There are 8 animals in each group.Except for the blank control group,all other groups were treated with traumatoplasty for Qi deficiency and blood stasis to create molds.After the operation was successfully performed,SD rats in the blank control and model control groups were given 0.9% saline gavage,and SD rats in the Astragalus-Safflower liquid group were given the corresponding doses of liquid for 21 d.After the last gastric gavage,1.the healing rate of the traumatic surface in each group was calculated;2.the morphological changes of the traumatic surface tissues in different groups were observed under microscope;3.phosphatidylinositol 3-kinase(PI3K)and protein kinase B(A)were detected in the traumatic surface tissues of each group,protein kinase B(Akt)and mammalian target of rapamycin(mTOR)protein expression levels in each group.Results: Comparison of trauma healing in each group:compared with the model control group,the trauma healing rate was significantly higher in the low and medium dose Astragalus-Safflower solution groups(P<0.05).2.Histomorphological changes of trauma:compared with the blank control group,the trauma tissues of the model control group showed significant inflammatory reactions;compared with the model control group,the trauma tissues of the low,medium and high dose Astragalus-Safflower solution groups were more mature and the trauma repair in the high dose Astragalus-Safflower solution group was the closest to that of the blank group.3.The expression levels of signaling pathway proteins were lower in PI3 K,Akt and mTOR in the traumatic tissue of the low,medium and high doses of Astragalus-Safflower solution groups compared with the model control group(P<0.05),and the lowest in the high dose of Astragalus-Safflower solution group.Conclusion:The "Astragalus-Safflower" pair can effectively promote wound healing and reduce scar growth in SD rats,and the high-dose solution is more effective than the low-dose solution.The mechanism of action is related to inhibition of PI3K-Akt-mTOR signaling pathway,inhibition of inflammatory response,and impact on tissue growth and differentiation of traumatized cells.