| Objective:The wound healing after anal fistula surgery is slow,and traditional Chinese medicine has advantages in the treatment of wound healing after anal fistula surgery through multi-channel and multi-directional adjustment during the wound healing process.This study investigated the therapeutic effect of Xiangpi Shengji ointment on wounds after anal fistula operation in rats and its correlation with PI3K/Akt/mTOR signal pathway,providing experimental basis for clinical promotion of Xiangpi Shengji ointment.Methods:SD male rats were randomly divided into four groups:sham operation group,model group,Xiangpi Shengji ointment group,and MEBO group,with 10 rats in each group.All four groups were used to create an anal fistula model using No.0 steel wire.After successful modeling,except for the sham operation group,anal fistula resection was performed under anesthesia,and the wound was kept open to form an infectious anal fistula postoperative wound that was "open,oozing blood,oozing fluid,and purulent secretion."In the sham operation group,the wire was removed and no dressing was changed.In the model group,normal saline was rinsed and iodine was disinfected.In the treatment group,wound dressing was changed with corresponding drugs for a total of 10 days.After 10 days,the wound healing rate and granulation tissue coverage rate of rats in the model group,the Xiangpi Shengji ointment group,and the MEBO group after anal fistula surgery were compared using conventional area detection methods;HE staining was used to observe the pathological changes of perianal tissues of rats in each group;The expression levels of bFGF,EGF,and VEGF in serum of rats in each group were detected by ELISA,and The expression levels of PI3K/Akt/mTOR signaling pathway related proteins in perianal granulation tissues of rats were detected and compared by WB.Results:Compared with the model group,the wound healing rate and granulation tissue filling rate of the Xiangpi Shengji Ointment group and the MEBO group significantly increased on the 3rd,7th,and 10th day of treatment(P<0.01);Pathological sections showed that in the sham operation group,there was less inflammatory cell infiltration,while in the other groups,there were different degrees of inflammatory cell infiltration,and different degrees of inflammatory repair type granulation tissue proliferation,collagen fiber regeneration,and vascular dilation were visible in the wound area.Pathological sections of the model group showed a large amount of inflammatory cell infiltration,significant vascular dilation,and significant vascular bleeding.Pathological sections of the Xiangpi Shengji Paste group showed fewer inflammatory cells,fibroblasts are mature and evenly distributed.Angiogenesis is visible,without significant vasodilation and bleeding.Pathological sections in the MEBO group show a small amount of inflammatory cell infiltration and vasodilation,and fibroblasts are visible in the dermis.The results of ELISA showed that the levels of bFGF,EGF,and VEGF in the serum of the model group were significantly lower than those of the sham operation group(P<0.01);Compared with the model group,the content of bFGF,EGF,and VEGF in the serum of the Xiangpi Shengji Ointment group was significantly increased(P<0.01),while the content of EGF,VEGF in the serum of the rats in the MEBO group was significantly increased(P<0.01).The WB test results showed that compared with the sham operation group,the expression of p-PI3K,p-Akt,p-mTOR,and p-p70 S6K protein in the perianal tissue of the model group was significantly decreased(P<0.01);Compared with the model group,the expression of p-PI3K,p-Akt,p-mTOR,and p-p70 S6K protein in perianal tissue in the Xiangpi Shengji ointment group was significantly increased(P<0.05).Conclusion:Xiangpi Shengji Ointment can effectively promote wound repair after anal fistula surgery,mainly manifested in shortening the time for wound repair,promoting wound angiogenesis,granulation growth,and fibroblast production,reducing inflammatory cell infiltration in the wound,and increasing the content of bFGF,EGF,and VEGF.Its healing promoting mechanism may be related to the expression of PI3K/Akt/mTOR signal pathway related proteins,which can upregulate the PI3K/Akt/mTOR signal pathway related proteins,activate this signal pathway,reduce the inflammatory reaction of wound after anal fistula surgery,promote the growth of wound granulation,and accelerate the process of wound repair. |
PDF Full Text Request