Exploring The Mechanism Of Shaoyao Decoction In Intervening In Rats With Ulcerative Colitis Of Endogenous Damp-heat Type Based On Network Pharmacology And Experimental Verificatio

Objective:To explore the potential mechanism of Shaoyao Decoction in the treatment of damp-heat ulcerative colitis(UC)with based on network pharmacology prediction and verified by animal experiments.Methods:TCMSP and UniProt database were used to screen the active components and related targets of Shaoyao Decoction,and GEO database was used to screen disease targets of UC.The intersection targets obtained by matching drug targets and disease targets were imported into STRING to construct a PPI network,and a"drug-compound-disease target" network was constructed with the help of Cytoscape software.GO functional enrichment analysis and KEGG pathway analysis were performed using R language.An animal model of damp-heat UC rat was established and intervened with Shaoyao Decoction to preliminarily verify the core targets and related pathways.DAI score was performed during the administration period after modeling;HE staining was used to observe the pathological changes of colon;ELISA was used to detect the content of motilin(MLT)in the plasma of rats to verify whether the model of damp-heat UC rats was successfully constructed and the content of serum inflammatory factors interleukin-6(IL-6)and interleukin-1β(IL-1β)in rats;Western blot was used to detect the expressions of related core genes MMP9,STAT3;immunohi stochemi stry(IHC)was used to detect the expression of NF-κBP65.Results:In this study,141 active components of Shaoyao Decoction in the treatment of UC were screened,and 69 targets were used.PPI screening and topological analysis were used to obtain 10 core genes,and GO and KEGG enrichment analysis was used to predict the key signaling pathways of Shaoyao Decoction in UC.The results of animal experiments showed that Shaoyao Decoction Shaoyao could increase the content of MLT in plasma of damp-heat induced UC rats,decrease the content of IL-6 and IL-1β in serum of model rats,and inhibit the expression of NF-κBp65,MMP9 and STAT3.Conclusion:Shaoyao Decoction can increase the content of MLT by regulating IL-6,IL-1β,MMP9,STAT3 and other targets,and interfere with the NF-κB signaling pathway,in order to treat damp-heat UC.