| Objective:This learn about ambitions to furnish theoretical and information aid for the modified decoction of Tounong Powder remedy of ulcerative colitis from the point of view of multi-component and multi-target action,and supply a new concept for "carbuncle primarily based treatment" of ulcerative colitis.Firstly,the network pharmacological technology was used to predict the effective ingredients,important action targets and signaling pathways of the modified decoction of Tounong Powder in the treatment of ulcerative colitis,and strive to identify potential action mechanisms,so as to determine the validity of the theory,select key targets as laboratory indicators of clinical trials.Secondly,The effectiveness and safety of the modified decoction of Tounong Powde therapeutic stage colon wet type UC are evaluated multifaceted after the following clinical trials.Methods:Network pharmacological analysis: The goal of all the energetic substances in the modified decoction of Tounong Powder was once retrieved from TCMSP database,and the applicable genes of UC have been got by using the use of Genecards,OMIM,TTD and Drug Bank databases.The frequent pursuits of pills and illnesses had been bought via evaluating the goal of the modified decoction of Tounong Powder with the disorder genes of UC,and the Venn creates maps used to be constructed.With the assist of Cytoscape 3.9.1 software,assemble the advantageous elements of the modified decoction of Tounong Powder and the frequent goals of tablets and diseases,and construct the visualization the modified decoction of Tounong Powder manage community mannequin to analyze the relationship between every node.Create a community plot(PPI)of the interplay of proteins in the String database,use the cytoscape 3.9.1 software program to open the PPI and installation the Cyto Hubba plug-in to meet it to create a community core.We use David database for route evaluation of GO and KEGG.Clinical verification: According to the inclusion,exclusion and exclusion criteria,60 eligible UC victims in the 2nd Anorectal Department of Anhui Hospital of Traditional Chinese Medicine from January 2022 to December 2022 had been screened,and 30 sufferers in every cure team(the modified decoction of Tounong Powder group)and manage team(Mesalazine group)have been installed via checking the random quantity table.Each 30 examples in eight weeks,the modified decoction of Tounong Powder installed oral treatment.The manipulate team used to be given oral remedy with mesalazine enteric-coated pills,in the course of which capsules affecting the statement of the therapeutic impact have been stopped.Record the modifications in medical signs of sufferers in the two groups and consider the therapy impact in phrases of scientific complete therapy effect,medicinal drug symptom integral,medication remedy impact evaluation,expanded Mayo rating,serum IL-6 and TNF-α stages exchange and safety.Results:Network pharmacology Part:(1)81 TCM components were integrated in the TCMSP database,5564 ulcerative colitis targets were obtained in the above disease database,and 220 drug targets were obtained after simplification,167 common targets were obtained for the modified decoction of Tounong Powder and ulcerative colitis.(2)The common targets of the modified decoction of Tounong Powder and ulcerative colitis were uploaded into the STRING database.Select human species to construct PPI map,and at the same time build network core to finally determine TP53,AKT1,TNF,IL-6,CASP3,EGFR,etc.as key targets.(3)GO and KEGG enrichment analysis was performed on the target proteins of the modified decoction of Tounong Powder in the treatment of UC: In GO analysis,818 cases of BP(biological process),92 cases of CC(cell component)and 151 cases of MF(molecular function)were obtained.KEGG evaluation in general targeted in rage pathway,AGE-RAGE signaling pathway,IL-17 signaling pathway,TNF signaling pathway,PI3K-AKT signaling pathway,P53 signaling pathway,etc.Clinical verification part:(1)The results of the modified decoction of Tounong Powder group and Mesalazine group on complete efficacy,enchancment of Mayo rating and inhibition of inflammatory response have been similar.(2)The ratings of TCM signs and symptoms(diarrhea,pus and blood stools,belly distension,stomach pain,anal burning,and posterior rigor and acute distension)in each corporations have been lowered after therapy(P<0.05),amongst which,the modified decoction of Tounong Powder group was once extra most reliable than Mesalazine group in enhancing stomach ache and distension(P<0.05).After treatment,there was once a statistical distinction in the whole ratings of TCM signs and symptoms between the two corporations(P<0.05),and the modified decoction of Tounong Powder group had extra benefits in assuaging TCM signs of UC.(3)The healing impact of the modified decoction of Tounong Powder group was once higher than that of Mesalazine group(P < 0.05).(4)Compared with Mesalazine group,the modified decoction of Tounong Powder group had greater security and no apparent aspect effects.Conclusion:(1)By using the network pharmacology,we have verified theoretically that the modified decoction of Tounong Powder has a therapeutic effect on ulcerative colitis.(2)Clinically,it has been clinically demonstrated that the modified decoction of Tounong Powder and mesalazine enteric-covered tablets is effective in treating ulcerative colitis.The treatment effects of the two groups in overall effect.In phrases of complete efficacy,extended Mayo rating and inhibition of inflammation,the therapeutic consequences of the two groups are comparable.In phrases of enhancing the major symptoms,efficacy and security of TCM symptoms,the therapy group has highlighted sure blessings of TCM,which can be utilized and in addition studied in scientific practice.Continuously give solid theoretical support and data support for "treating ulcerative colitis from carbuncle". |
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