Study On Biodegradation And Pathway Of 1-Amino-4-bromoanthraquinone-2-sulfonic Sodium

Bromamine acid (l-Amino-4-bromoanthraquinone-2-sulfonic sodium) called ABAS for short is an important intermediate in production of reactive dyes and acid dyes. ABAS manufacturing wastewater exhibits very high COD and color. In Chapter One, different treatments of ABAS wastewater, especially biological treatment have been reviewed. Therefore, the significance and background of this study in the paper has been showed.HP3 screened from active sludge could efficiently degrade ABAS. The dynamic characters of ABAS degraded by HP3 were studied. By analysis of intermediates and products of ABAS degraded by HP3, the possible pathway of ABAS degradation was discussed. The characters of extracellular enzymes produced by HP3 were also examined in this paper. The main conclusions are drawn as follows:1. HP3 identified as Zoogloea sp. is an effectively strain. Under the optimal degradation condition and ABAS concentration of 200~1200mg/L, the ABAS degradation followed negative exponential model. With the ABAS concentration increasing, the ABAS degradation was greatly regressed. Temperature was the most important factor influencing ABAS degradation. The functions of specific growth rate and temperature, degradation rate of ABAS and temperature were constructed respectively.2. Addition of metal compounds, NaCl, different carbon and nitrogen sources could enhance or inhibit the degradation rate of ABAS. Glucose could cooperate with (NH4)2SO4 to enhance the degradation rate of ABAS. The optimal addition of glucose and (NH4)2SO4 were discussed in this paper.3. The possible pathway of ABAS degraded by Zoogloea HP3 was that ABAS was first cleaved to produce o-phthalic acid and products. The former wasfurther degraded and the composites of the latter were 2-amino-3-hydroxyl-5-bromobenzenic sulfonic sodium and 2,3-dihydroxyl-5-bromobenzenic sulfonic sodium.4. ABAS could be decolored by extracellular composite enzymes secreted by Zoogloea HP3. The degradation place of o-pathalic acid was in-cell. Compared with the degradation of o-pathalic acid, the decoloring of ABAS controlled the degradation of ABAS.5. C. I. Reactive blue KN-R, Alizarin brilliant green G and Hostlam blue R could not be degraded by Zoogloea HP3. Furthermore, ABAS degradation was regressed under the exisitence of the above dyes. l-Aminoanthraquinone-2-sulfonic sodium, 1,4-dihydroxylanthraquinone-2 -sulfonic sodium, 1,4,5,8-tetrahydroxylanthraquinone, anthraquinone, aniline, phenol, catechol and o-phthalic acid could be degraded by Zoogloea HP3. Aniline was the most degradable substrate among ABAS, aniline, phenol, catechol and o-phthalic acid. However, benzenesulfonic sodium and p-amino benzenesulfonic sodium were not?degraded by it.Overall, this paper is proved to be meaningful in both theoretical explanation and practical application of biodegradation of anthraquinone resembles .