| Based on the current achievement on natural product, a general review ofnatural product chemistry and its application on investigation protocol, resourcesprotection, analysis technology, biology and chemistry synthesis was made. Furthermore, this review also covers the preparation and detection of Tripterygium \vilfordii.First, several extraction and preparation technologies of the alkaloid compounds, the diterpenoid triepoxides compounds and the saccharide compounds were studied. Two column chromatography methods for the separation wilforine and wilfordine , the triptolide and triptonide were developed, respectively.Second, a micellar electrokinetic capillary chromatography (MEKC) method has been established for the identification and determination of diterpenoid triepoxides in Chinese herb Tripterygium wilfordii Hook F and its preparations. The detailed investigations of the influence of SDS concentration, pH, boric acidand borax concentration were carried out. From the experiments, the MEKC was found to exhibit good accuracy, precision and repeatability.A capillary electrophoresis method has been established for the determination of triptonide and triptolide in Multi-glycosides Tripterygium wilfordii tablets. Capillary separation was carried out by using 0.01mol L-1 borax + + 0.02mol L-1 SDS (pH =7.3) as the buffer, 20kV applied voltage and 301K temperature.A method for the separation of wilforine and wilfordine in Tripterygium wilfordii Hook F based on nonaqueous capillary eletrophoresis has been developed. The optimum separation conditions are as follows: voltage 15kV, the temperature was kept at 301K and the running medium consisted of 20mM ammonium acetate and 3mM ammonium hydroxide in methanol. The feasibility of this method was demonstrated by the determination of wilforine and wilfordine in Tripterygium wilfordii Hook F.A capillary electrophoresis method has been established for the zolmitriptan of chiral drug.
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