Experimental Study On Changes Of Immune Molecule Following Corneal Alkali Burns, Acid Burns And Thermal Burns And The Treatment

Ocular burns is one of the most severe corneal injuries, and is mostly involved in criminal cases and industrial accidents. Although the reason of wounding is different, the ocular burns frequently lead to the necrosis of tissue, the formation of scar tissue, recurrent corneal erosions. At present, though the mechanism for this devastating process remains largely unknown besides the direct injury of chemical matter and heat, we know it correlates with immune mechanism, especially alkali burn. Immune system is a complicated network structure consisting of immune cells and immune molecules. The immune cells and immune molecules alter in different degree after ocular burns, expressing the excessive inflammation and low immune reaction. This study reveals the mechanism of immune disturbance after ocular burns from the aspects of immune molecules.Part I Variety of corneal protein 's antigenicity following corneal alkali burns, acid burns and thermal burns and the study of differential display on geneThere is not immune reaction in normal cornea between corneal protein and tissue because the corneal protein does not touch the lymphatic circulation. But alkali burns cause the production of antibodies of denatured corneal protein. Hanan produced alkali burns in the right eyes of 63 mice. Three weeks later the left eye of each mouse was similarly burned. All eyes were followed up weekly for 4 weeks after the burn and were graded according to the severity of the lesion. The lesions developed much faster and were more severe in the left eyes. These results suggested that the body's immune mechanism participated in the pathogenesis of alkali corneal burns and adversely affectedthe development of this condition. Kao found Western blot analysis demonstrated a production of heterogeneous antibodies in a majority of the rabbits (11 of 14) to various denatured corneal proteins (between 80 kDa and 25 kDa) at 5 weeks of alkali burns. Antibodies to corneal protein were detected after alkali burns one week later and disappeared after six weeks. Corneal alkali burns stimulate a response of the humoral immune system which produces the specific antibodies against denatured corneal proteins. Corneal alkali burns are characterized by persistent inflammatory response and recurrent epithelial erosions. Its pathologic trauma and course of diseases are more serious than the acid burns and thermal burns. So we think the pathologic mechanism of alkali burns different from that of acid burns and thermal burns. It needs father study on investigating if all kinds of ocular burns can produce denatured corneal proteins.Protein is the essential material to represent the essence of living and its synthesis is dominated by RNA. We thought the abnormity of mRNA after ocular burns led to the change in corneal protein's synthesis. We detected the differential fragment or change in expression of gene by DDRT-PCR after corneal alkali burns, acid burns and thermal burns in the rat and cloned them. We hoped to illustrate the base of producing corneal denatured protein from view of gene.1. Variety of corneal protein 's antigenicity following corneal alkali burns, acid burns and thermal burnsPurposeTo detect the change of corneal protein and determine if there is any new denatured protein and detect their antigenicity following alkali burns, acid burns and thermal burns. Method1 .Twenty-eight rabbits weighting 2.0-2.5 kg were divided into the groups of alkali burns, acid burns, thermal burns and the control respectively.2.The alkali injury was created by applying an 8-mm filter disk soaked in 1 N NaOH on the center of the cornea for 1 min, and then the acid injury by 1 N H2SO4. The thermal injury was created by applying an 8-mm iron disc heated 30 sec on the center of the cornea for 30 sec.3.Serum was collected weekly and subjected to Western blot immunostaining to detect antibodies against denatured corneal proteins.4.The extracted corneal protein was separated by SDS-PAGE and analyzed byWestern-blot to determine its ant...