Mechanism Study On Fallopian Tubeinflammatory Obstructive Infertility Model Ratstreated By Yankening

To study on the mechanism of Yankening treating fallopian tube inflammatory obstructive infertility(FTIOI),the 96 rats were inoculated mixed bacterium to copy the model, which were divided into the normal group,the model group, the TCM controlled group,the lower dosage group, the middle dosage group, the high dosage group, while the every group was divded into two time group which were 15 days and 30 days.HE staining was used to observe the change of fallopian tube in morpholog ,while to observe the ultrastructure with electron microscope,to detect the cell apoptosis with TUNEL staining,to detect BCL-2,EGFR and ICAM-lmRNA with immunohistochemistry and hybridization in situ, and to detect IL-l,IL-6,TNF-ain serum with ELISA.The results showed that: (1) To compared with the model group, the TCM controlled group,the lower dosage group, the middle dosage group, the high dosage group can restrain the cell apoptosis,relieve hyperemia, edema, adherence and granuloma,and accelerate tissue repair; (2)The high dosage group can improve the expression of BCL-2 mRNA and protein;(3)The high dosage group can decrease the expression of ICAM-lmRNA and protein;(4)The high dosage group can decrease the expression of EGFR mRNA and protein; (5)The high dosage group can decrease the contentof IL-l,IL-6 and TNF-ain serum.The conclutions: (1)Mixed bacterium inoculated can copy the model; (2)The middle dosage group, the high dosage group can restrain the cell apoptosis,relieve hyperemia, edema, adherence and granuloma,and accelerate tissue repair;(3)By improving theexpression of BCL-2 mRNA and protein,the Yankening can anti-apoptosis;(4)By decreasing the expression of EGFR mRNA and protein, Yankening can have anti-inflammatory action; (5)The high dosage group can decrease the contentof IL-l,IL-6 and TNF-ain serum.