Study On Lipid-regulation And Anti-atherosclerosis With Maixinkang And Intervention In The Atherosclerosis Of Apolipoprotein E Gene Knockout Mice

Objective: 1. Clinical research: to observe the effects of Maixinkang capsule on patients with hyperlipidemia and carotid atherosclerosis. 2. Experimental research: to observe its effects on blood lipids and atherosclerosis of rats with high fat diet and mice with the knock-out apolipoprotein E gene.Methods: 1. Clinical research: patients with hyperlipidemia and carotid atherosclerosis were divided randomly, to observe the effect of Maixinkang, Xuezhikang and Lovastatin on blood lipid, endothelium-dependent diastolic function of brachial artery, IMT aherosclerotic plaque and clinical symptoms after treating for 6 months. 2. Experimental research 1: to reproduce high fat diet rat models with hyperlipidemia and atherosclerosis, to observe the effects of Maixinkang, Xuezhikang and Lovastatin on blood lipid after 30 days, and the variations of MDA, SOD, NO, TNF- a and viscosity of blood And observe the pathological and morphologic changes of aorta to give image analysis to IMT and smooth muscle cells. Experimental 2: 6-week-old ApoE(-/-) mice were divided randomly, to observe the effects of Maixinkang, Xuezhikang and Lovastatin on blood lipid after 34 weeks.To observe the slice of aorta and heart with microscope. Some aorta was observe with electro-microscope and to detect the mRNA expression of NF-KB, MMP-9. To take a morphological observation with liver and detect the changes of calcium in cells. To culture liver cells with 2-week-old ApoE(-/-) mice, and observe the effects of normal Saline, Maixinkang, Xuezhikang and Lovastatin on calcium and JC-1 of mitochondria.Results: 1. Maixinkang could reduce blood lipid level of patients with hyperlipidemia, high fat diet rat and ApoE(-/-) mice. 2. Maixinkang could relieve the carotid atherosclerosis plaque, improve endotheliurn diastolic function and clinical symptoms. 3.Maixinkang could reduce serum MDA, TNF- a level and elevate SOD, NO level, and lower blood viscosity. 4. Maixinkang could have an anti-proliferation of smooth muscle, anti-athrosclerosis and plaque stabilization effects. 5. Maixinkang could reduce the mRNA expression of NF- B and MMP - 9 of aorta. 6. Maixinkang could protect the morphological structure of aorta, myocardium and liver, especially super-microstructure. 7. Maixinkang could reduce calcium overloading of cultured liver cells. 8. Maixinkang could regulate the expression of JC-1 of mitochondria in cultured liver cell. 9. Maixinkang was superior to Xuzhikang and Lovastatin in improving clinical symptoms and some other objective indexes.Conclusion: Our research indicated that Maixinkang had an exact effect of reducing blood lipid, protecting vascular endothelium, anti-oxidation, anti-inflammation, reduction of viscosity of blood, anti-proliferation of smooth muscle cells, protecting myocardium and super-microstructure, regulating the mRNA expression of NF-icB and MMP -9, reducing calcium overloading, regulating membrane potential JC-1 of mitochondria, so it could regulating blood lipid, anti-atherosclerosis and stabilize atherosclerotic plaque, it had great value in treating and preventing hyperlipedemia, atherosclerosis,ACS, et al.