The Research On Pharmacodynamic Mechanism Of NLST In Treatment Of ICVD

1 Objectives Stroke is the most common disease in the aged, which has damaged the health of human beingseriously. The thesis discussing the relatively aspects pertain to the relation between the stasis,waterand toxic and IVCD, and the pharmacodynamic mechanism of NLST in the develepmenting ofIVCD. This topic guided by the rational of TCM analysised the episode regularity of ischemic stroke,and realized the collection of stasis, water and toxic in the brain is the focus pathology in the acuteperiodofstroke,andgavea suggestionthatbloodactiviting diuresis,detoxicate is the keywayinthetreatment of cerebral edema caused by ischemia. It discussed the diuresis function of NLST from itseffects on the water volume in the brain tissue blood brain barrier permeability neuropeptide andMMP. And discussing the detoxication of NLST by observing the changes of TNF-α IL-1 in theblood serum of patients and therapeutic mechanism of NLST in the nerve function remodeling fromthe exprssion of neurotrophic factor and proliferation of nerve stemcell. 2 Method 2.1The focal cerebral ischemia model was induced by photochemistry in rats, all experimentalrats were divided into NLST treatment group, model control group, pseudoperarion group, vacuitygroup .NLST was successively poured into rats' stomach for 4days begun in 3 hours after cerebralischemia was induced. Rats' weight and clinical score were detected each day. The brain wereexcised in the 5th day of postoperation, HE stain and TTC stain were done for measuring volume ofinfarction and observing pathologic change.2.2 By adopting MCAO in rat models, achieve MCAO3h/R24h, observing nerves function scoreand water volume in the brain tissue of each group. Observing BBB permeability variant by themethod of Evans blue fluorometry, and Observing cerebrum -EP Protein Expression of processedMCAO/R rats by the method of immunohistochemistry, Estimate the expression of MMP-9mRNAby RT-PCR. 2.3 By constructing rats blood stasis model, and analyse blood, observe the affect of NLST tothe blood coagulatlon-Fibrinolysis systemof blood stasis rats. 2 4 To make RIA by liquid phase competition, Observe the influence of TNF-α and IL-1 inthe patients' blood serum between treatment group and contrast group in clinical pretherapy andpost-treatment by NLST. 2 5ObserveExpressionof5thdaybFGFand10thdaynestinproteinoffocalcerebralischemiainduced by photochemistry in rats of NLST treatment group, model control group, pseudoperariongroup, vacuity group by immunohistochemistry. 3 result 3 1 NLST can reduce the volume of infarction of rats induced by photochemistry. The volume英文摘要 5of infarction of treatment group was 7.32±2.03mm3, the volume of the contrast group is16.01 2.62mm3, a sharp contrast can be observed between the two group the volume of infarction(p<0.001). thepathologic change of treatment group is relieved than constract group after the drug. 3 2 MCAO3h/R24h in rats, water volume and EB content in the brain tissue in the side ofischemia ofcontrol group(79.24 0.61%) 4.78 0.72ug/g ,whichhavea higher volumethanthevacuity group(73.43 0.57%) 2.07 0.15ug/g ,water volume andEBcontentinthebraintissueinthesideofischemiaofTreatmentgroup(76.85 0.87%) 3.52 0.63ug/g issignaificantlyreduced,have a significant difference compared to model control group(P<0.001). pseudoperarion group hadno significant difference comparedto vacuity group. water volume and EB content inthe brain tissueinthe sideofnon-ischemiaofcontrl group(75.59 0.48%) 3.24 0.57ug/g isalsohigher thanthevacuity group(P<0.001). MCAO3h/R24hinrats,theexpressionof -EPproteininthebraintissue intheside ofischemiaof model control group obviously increased, expressed by glial cell primary. the expression of -EPprotein in the brain tissue in the side of ischemia of treatment group is decreased obviously, have asignificant difference compared to contr...