Anti-Malassezia Actions Of Combination With Ketoconazole, Terbinafine And Chinese Herbal Components

With realization and acknowledgement of skin diseases related to Malassezia species, so it is very important for the whole society to develop anti-Malassezia agents with high-effective and low-adverse. The study is divided into five parts, discuss the effect about anti-Malassezia agents that Chinese herbal components combination with western medicine, mainly including the susceptibility testing of Malassezia species in vitro, the ultrastructural and cellular cycle of changes of Malassezia species on the cellular and sub-cellular level, experimental animal models. Its result showed that there were difference among species distribution between several kinds of skin diseases related to Malassezia species. The susceptibility testing of Malassezia species in vitro were taken by NCCLS M27-A microdilution method. It was selected that the combination ketoconazole plus thymol has better anti-Malassezia activity than single agent againstMalassezia species. It also demonstrated that the combination ketoconazole plus thymol has better anti-Malassezia activity than single agent against Malassezia species on the cellular level that supported by way of TEM and FCM. The possible target of thymol against Malassezia species was cellular wall or membrane. Meanwhile, it further demonstrated that results of the susceptibility testing of Malassezia species in vitro by the results of experimental animal models. This research provides theory basis for further develop anti-Malassezia agents with high-effective and low-adverse. Meanwhile, these results provided that referred proofs about experiment standards of the susceptibility testing of Malassezia species in vitro.