Effect Of Calcium Antagonist Amlodipine On Apoptosis Of LLC-PK1 Cell

It was suggested that toxic agent-related apoptosis may lead to renal tissue damage and lose of renal function. However, the mechanism of the apoptosis of renal cells induced by intoxication remains unclear. Although a few reports suggest that expression of some apoptosis co-gene may be involved in the process of renal apoptosis, it is unknown whether the intracellular calcium variation is related to this process .In this study, we examined the influence of different concentration of gentamycin(GM) in inducing apoptosis of LLC-PK1 cells and the effect of calcium antagonist amlodipine on intracellular calcium levels of apoptotic cells. The relationship among LLC-PK1 apoptosis and intracellular calcium levels and gene expression were also investigated.LLC-PK1 cells was treated in different groups: normal (without GM ); with GM (GM<1mM, G=2mM,4mM,8mM,16mM); with amlodipine(A); with GM(8mM) plus A(1, 10, 100pg/ml). Apoptosis of LLC-PK1 cells was identified by light microscopy,electromicroscopy, argaros gel electrophoresis and TUNEL method. Cell membrane blebbing was seen under inversive microscopy. "Ladder" and nuclear margination and condensation were also identified separately. Quantitative analysis of...