Experimental Studies On The Role Of Subchondral Bone Variation In Osteoarthritis And The Intervention Effect Of Diphosphonate

Partâ… Biomechanical variation of subchondral bone and the intervention effect of diphosphonate in osteoarthritis.Experimentâ… : Biomechanical variation of subchondral bone and the intervention effect of diphosphonate in instable knee joint of early stagObjective Observing the biomechanical changes of subchondral bone of the rabbit instable knee joint at its early stage and the intervention effect of diphosphonate ,to investigate the role of biomechanical changes of subchondral bone at the early stage of osteoarthritis in initiation and progression of osteoarthritis.Methods 50 male New Zealand white rabbits were divided randomly into three groups according to random digits table: the control group (n=10), the model group (n=20). the diphosphonate group (n=20).. Hulth model of rabbit instable knee joint(transecting anterior cruciate ligament and medial collateral ligament,resecting medial meniscus) was achieved in the right knee joint .The rabbits in the diphosphonate group were injected intravenously with Pamidronate Disodium at 1mg/kg immediately after surgery ,and the medicine was used again one month later;The rabbits in the control group and in the the model group were injected intravenously with normal saline of the same volume at the same time.10 rabbits from the model group , 10 rabbits from the diphosphonate group and 5 rabbits from the control group were sacrificed by aeroembolism at 2 week and 10 week following surgery. The subchondral bone of main loading area of the medial tibia plateau in the right knee was harvested and the biomechanical parameters were tested at the same time to detect its loading-displacement, stress-straining, compressive strength and...