The Investigation Of Human Pancreases Islets Transplantation

Objective To contrast methods of cutting human pancreases from cadaver donors for islets transplantation, and to discuss the relationship between activity of islets and methods of cutting human pancreases. To establish a new method of cutting human pancreases from cadaver donors for islets transplantation, and to discuss the relationship between activity of islets and the cold schema preservation time after hypertonic citrate adenine solution perfused to investigate the relationship between the living of islet and HLA typing in the donor and recipients To discuss the bleeding from the portal vein needle puncture site and infection in liver after the islets transplantation in swine.Methods Hypertonic citrate adenine solution was perfused into aorta, kidney-pancreases and kidney-pancreases-liver, were cut together, kidney-pancreases-liver were cut separateness, digestion of pancreases was done with collagenase P, then stained the islets, then contrast activity of islets with different methods 1,500-2,000ml of hypertonic citrate adenine solution was perfused into aorta, kidey-pancreases-duodenum-spleen were cut together, digestion of pancreases was done with collagenase P, then stained the islets put the islet which have known HLA typing into the blood whose HLA typing is compatible for it, the blood whose HLA typing is incompatibility for it. Observe the variability of platelets, neutrophils, monocytes and lymphocytes in the blood after cultivates 1 hour. The varieties of...