NGF Controlled Release System Enhances Rat Sciatic Nerve Regeneration After Electric Injury

Background and Research StrategyIt was conclusively confirmed that nerve growth factor could enhance peripheral nerve regeneration after injury. However, due to the short half-life action of NGF and suffered deactivation easily from humidity and acidity or alkalinity, the clinical application is difficult consequently. It is necessary to search a new reasonable way of drug administration therefore.The incidence rate of nerve electrical burn is higher recently. There has no efficient therapy scheme in clinic at present. It is important to search an effective way to heal the impaired nerve as soon as possible to shorten the recovery time.Thus, our experiment protocol is that to develop a NGF delivery system for use in repair of peripheral nerve after electric injury and we hypothesize that it would provide localized release of NGF in an active way to enhance peripheral nerve regeneration.Based on above hypothesis, we conduce the researches as follows:Firstly, we prepared a NGF controlled release system. We evaluated the ability of the delivery system to prolong the release of NGF in absence of cells by Sandwich ELISA and we determined if the delivery system could delivery bioactive NGF in a controlled manner and evaluated the necessity of the composition by DRG culture model.Then we design an animal experiment model of peripheral nerve electric injury which mimicry clinical electric burn. By using light microscope and electron microscope to observe the changes of nerve morph and using SFI and NCV to observe the changes...