A Research On The Expression Of The Costimulator CD₂₈ Of CD₈～+T Lymphocytes From Peripheral Blood In Non-acute Infection Periods Of The Recurrent Respiratory Tract Infection's Children With Different Syndrome Types

Aims:Through the measurement and analysis of the CD₈⁺,CD₂₈⁺,CD₈⁺ CD₂₈⁺ andCD₈⁺ CD₂₈^- expression of T lymphocytes from peripheral blood in qi deficiency syndromeof lung and spleen(QDSLS) and qi and yin deficiency syndrome of lung andspleen(QYDSLS) of the recurrent respiratory tract infection's children in non-acuteinfection periods to discuss its characteristics of expression, clinical implications andexplore the potential objective foundation in Syndrome Differentiating of ChineseMedicine.Methods: Choosing the eligibility samples according to the diagnostic, eliminate andinclusive criteria, immunodetections including CD₈⁺,CD₂₈⁺,CD₈⁺ CD₂₈⁺ and CD₈⁺ CD₂₈^-of RRTI children of QDSLS and QYDSLS in non-acute infection periods by flowcytometry in T lymphocytes from peripheral blood are compared with the control group.Statistical analysis was performed by SPSS/PC10.0 system software.Results:1. The expression of CD₈⁺ is low in T lymphocytes in both QDSLS and QYDSLSgroups, p＜0.01 show significant differences between the groups QDSLS and control group.No significant difference (p＞0.05) was found in groups of QYDSLS and controlgroups,and the same result to the QDSLS and QYDSLS groups.2. The expression of CD₂₈⁺ is low in T lymphocytes in both QDSLS and QYDSLSgroups,and show significant differences compared with the control group (p＜0.01),difference (p＜0.05) was also found in QDSLS and QYDSLS groups.3. The expression of CD₈⁺ CD₂₈⁺% is low in T lymphocytes in both QDSLS andQYDSLS groups,and in the QYDSLS group it is lower than that of QDSLS group.Significant difference (p＜0.01) was found in each two groups.4. The expression of CD₈⁺ CD₂₈^-% is high in T lymphocytes in both QDSLS andQYDSLS groups,and in the QYDSLS group it is higher than that of QDSLS group.Significant difference (p＜0.01) was found in each two groups.5. The expression of CD₈⁺ CD₂₈⁺ is low in T lymphocytes in both QDSLS andQYDSLS groups,and in the QYDSLS group it is lower than that of QDSLS group.Significant difference (p＜0.01) was found in each two groups.6. The expression of CD₈⁺ CD₂₈^- in T lymphocytes in both QDSLS and QYDSLSgroups has no significant difference (p＞0.05) compared with the control group.But in QYDSLS group it is higher than that of QDSLS group and has significant difference(p＜0.01).7. In the QDSLS group, Multivariate regression analysis (MRA) indicates the marks ofTraditional Chinese Medicine syndromes(Y)is correlated with CD₈⁺ CD₂₈⁺% (C)(p＜0.01)and CD₈⁺ CD₂₈^- (E,) (p＜0.05), the correlation formulas can be expressed as Y=16.976-0.201C and Y=15.781-0.190C+0.07164E. Y is also correlated with CD₈⁺ CD₂₈^-%(H),the correlation formulas are Y=16.976-0.201 (100-H) and Y=15.781-0.190 (100-H)+0.07164E.8. In the QYDSLS group, MRA shows Y is correlated with C (p＜0.01),the correlationformula is Y=17.199-0.293C. Y is also correlated with H, the correlation formula isY=17.199-0.293 (100-H).Conclusions:1. Compared with the control group,the expression of CD₈⁺ is not increased in Tlymphocytes from peripheral blood in QDSLS and QYDSLS groups of the RRTI's childrenin non-acute infection periods, so the opinion "In RRTI,the expression of CD₄ isinduced, while CDs is increased, and the ratio is also induced" need further research,as itwas regarded as the norm to valuate the clinical effectiveness.2. In non-acute infection periods,in QDSLS and QYDSLS groups of the RRTI'schildren,the expression of costimulator CD₂₈ is induced, the pathway of the costimulationis abnormal on activating T lymphocytes;The expression of CD₈⁺ CD₂₈^- is increased in Tlymphocytes from peripheral blood while CD₈⁺ CD₂₈⁺ is induced indicates the imbalanceof CD₈⁺ lymphocyte subgroup.This may be one of the Pathogenesis of RRTI.3. The expression of CD₈⁺ CD₂₈⁺ in T lymphocytes is a potential indicator to reflectthe RRTI children's immune function in non-acute infection periods, and the expression ofCD₈⁺,CD₂₈⁺,CD₈⁺ CD₂₈⁺ and CD₈⁺ CD₂₈^- is helpful to the Traditional Chinese MedicineSyndromes Differentiating of QDSLS and QYDSLS in non-acute infection periods ofRRTI,and can be the objective indicator of the character of the different syndrome types.4. Alteration expressions of CD₈⁺ CD₂₈⁺% and CD₈⁺ CD₂₈^-% are correlated with themarks of Traditional Chinese Medicine syndromes of QDSLS and QYDSLS,it could bebetake as an assessment method in clinical therapeutic effects with the advantage of simpleand economic.