Mediterranean's Intended Branches Bacteria Acid Bacteria (amycolatopsis Mediterranei) U-32 Genetic Operating System To Establish

Amycolatopsis mediterranei U-32, an industrial producer of rifamycin SV, is difficult to be transformed. Factors affected the transformation efficiency, especially the culture conditions, were optimized for the transformation of this strain using DNA of actinophage (MMR1. Employing electro-competent cells prepared from early stationary phase mycelia cultured in MYM, a specially modified YEME medium and optimized electroporation conditions, a transformation efficiency of 5x105 pfu/(g phage DNA was obtained.