The Experimental Research Of Effects Of Shenqi Compound Recipe On Angiotensin â…¡ And Its Receptors In GK Rats With Macrovasculopathy

Objective:To elvaluate the therapeutic effect of ShenQi compound recipe(SQCR) on the diabetes mellitus macrovasculopathy GK rat,which has the function of tonifying Qi and Yin and promoting blood circulation to dissipate stasis,in the level of gene, molecular and cell leve.To demonstrate the mechanism of SQCR for diabetes mellitus macrovasculopathy from the view of angiotensin and its recepors.Methods:73 GK male rats were divided into four groups randomly:respectively 18 in GK group, drenched normal saline 5ml·kg-1·d-1,19 in model group, drenched normal saline 5ml·kg-1·d-1,18 in atorvastatin groups, drenched atorvastatin calcium suspension 1.6mg·kg-1·d-1,18 in SQCR group, drenched SQCR suspl 1.44g·kg-1·d-1. 18 SPF Wistar rats in the control group was drenched normal saline 5ml·kg-1·d-1. In order to build the diabetes mellitus macroangiopathy model, the model, atorvatatin, SQCR groups were administered with the Nω-nitro-L-arginine methyl ester(L-NAME) 0.1mg·ml-1·d-1 in the drinking water for 35 days. After sacrifice, detect the blood glucose, blood fat, blood secum C reactive protein (CRP) and AngiotensinⅡ;dectect the angiotensinⅡtype 1 receptor(AT1R)mRNA expression in the abdominal aorta vessel wall by real-time PCR; dectect the integral optical density of AT1R and AT2R in the abdominal aorta vessel wall by immunohistochemistry;detect the intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) by western blot.Result:The typical atheromatosis pathoalteration was observed in model group by light microscope in arteriae aorta appearance. Compared with other groups, the blood glucose, fat, CRP and AngⅡraised up in model groups (P<0.05 or P<0.01). Increased body weight, degraded blood glucose, fat, CRP and AngⅡand relieved inflammatory reaction was observed in the GK rats treated by SQCR. in addition, the SQCR can also downregulate the AT1RmRNA expression, decrease the synthesis of AT1R, enhance the AT2R, decrease the ICAM-1 and VCAM-1 in abdominal aorta vessel wall.Conclusion:SQCR could delay the development of diabetes mellitus AS by improving the growth, gaining weight, depressing blood glucose and blood fat, degrading blood CRP and AngⅡ.One of the possible treatment mechanisms in molecular biology is to decrease the AngⅡin the surem,downregulate the expression of AT1RmRNA and synthesis of AT1R,and increase the synthesis of AT2R in vessel wall.