The Experimental Research Of ShenQi Compound Recipe To PI3-K/Akt Signal Transduction In The Diabetes Mellitus Macroangiopathy

Objective:To observe the influence of PI3-K/Akt signal transduction with ShenQi compound recipe(SQCR) in the diabetes mellitus macroangiopathy GK rat abdominal aorta vessel wall. And to evalute the contribution of SQCR with the function of tonifying Qi and Yin and promoting blood circulation to dissipate stasis to the signal transduction passway. Research the therapeutic mechanism of prevention and cure to the diabetes mellitus macroangiopathy and the reaction mechanism of the organism with Traditional Chinese Medicine (TCM).Methods:Divided 73 GK male rats into four groups randomly: respectively 18 in GK group, drenched normal saline 5ml·kg-1·d-1,19 in model group, drenched normal saline 5ml·kg-1·d-1,18 in atorvastatin groups, drenched atorvastatin calcium suspension 1.6mg·kg-1·d-1,18 in SQCR group, drenched SQCR suspl 1.44g·kg-1·d-1.18 SPF Wistar rat act as the control group, drenched normal saline 5ml·kg-1·d-1. In order to build the diabetes mellitus macroangiopathy model, admin the Nω-nitro-L-arginine methyl ester(L-NAME) 0.1mg·ml-1·d-1 in the drinking water for the model, atorvatatin, SQCR groups with the administration simultaneously. Treated for 35 days, detect the blood glucose, blood fat, blood secum C reactive protein (CRP) and insulin, and the PI3-KP85a,Akt,eNOS mRNA expression in the abdominal aorta vessel wall by real-time PCR.Result:The typical atheromatosis pathoalteration is observed in model group by light microscope in arteriae aorta appearance. Compared with other groups, the blood glucose, fat, CRP and INS raise up in model groups (P<0.05 or P<0.01), and the HOMA-IRI highten too (P<0.01). Increased body weight, degraded the blood glucose, fat, CRP and INS, the GK rats gained improved glycometabolism and fat metabolism, and relieved Inflammatory reaction with insulin resistance. At the same time, the SQCR can also restrain the PI3-KP85a, Akt and eNOS mRNA expression in abdominal aorta vessel wall.Conclusion:SQCR could delay the development of the diabetes mellitus AS by improving the growth and development, gaining weight, depressing blood glucose and blood fat, degrading blood CRP, relieving IRI. One of the possible curing mechanisms in molecular biology is inhibiting the neovascularization for atheromatous plaque by restrain the expressing of PI3-K-Akt-eNOS mRNA in vessel wall.