Experimendal Study Of Bilogical Difference Of Molecular Hemorheology In Three Blood Stasis Model And Relation Of Prescription And Syndrome About The Cold Stimulation Blood Stasis Model

Objectives:To investigate biological difference of hemorheology of three kinds of blood stasis model from molecular level of red cell membrane. There were cold stimulation blood stasis model, starvation plus exhausted swimming blood stasis model and acoustics, optics, electricity complex stimulus plus clamp tail blood stasis model. Shaofu Zhuyu decoction, Buyang Huanwu decoction and Danshen decoction were applied to reverse approve the biological characteristics of red cell membrane of cold stimulation blood stasis model.Methods:The rats were observed in this article.(1) Dipping in ice water to simulate cold stimulation blood stasis model. Starvation plus exhausted swimming to simulate qi deficiency blood stasis model. Unpredictable acoustics, optics, electricity complex stimulus plus clamp tail irritation to simulate qi depression to blood stasis. After stimulation continual for two weeks, the token of cold stagnation blood stasis, qi deficiency blood stasis and qi depression blood stasis were appeared. The whole blood viscosity, erythrocyte deformability, blood sedimentation K value, four kinds of thrombin, plasma cholesterol and triglyceride were determined after taking blood from carotid. Then erythrocyte membrane were prepared to determine Na+-K+-ATP enzyme activity, the content of sialic acid, hydrosulphonyl, cholesterol, MDA and activity of SOD.(2) The cold stagnation blood stasis rats were intragastric administrated high and low dose Shaofu Zhuyu decoction, Buyang Huanwu decoction and Danshen decoction for two weeks. Except the above-mentioned index were determined, the structure of membrane skeleton were observed under laser scanning confocal fluorescence microscope. The constituent of membrane protein and percentage composition were determined.Results:(1) Compare with the blank group, the whole blood viscosity, blood sedimentation K value and the content of FIB of cold stagnation blood stasis group is increasing significantly. The erythrocyte deformability is decreasing and APTT is curtailing significantly. SOD and Na+-K+-ATP enzyme activity of erythrocyte membrane are decreasing and the content of sialic acid is decreasing (P<0.05or P<0.01); Under high shear the whole blood viscosity and erythrocyte deformability of Starvation plus exhausted swimming group is decreasing. APTT and PT is curtailing significantly. The content of plasma cholesterol is decreasing distinctly. SOD and Na+-K+-ATP enzyme activity of erythrocyte membrane are decreasing significantly (P<0.05or P<0.01); Compare with the blank group, APTT of unpredictable acoustics, optics, electricity complex stimulus plus clamp tail irritation group is curtailing significantly. SOD and Na+-K+-ATP enzyme activity of erythrocyte membrane are decreasing. The content of sialic acid is decreasing and MDA is increasing (P<0.05or P<0.01).(2) Compare with the cold stagnation blood stasis group, under low shear the whole blood viscosity is decreased significantly after i.g. Shaofu Zhuyu decoction, Buyang Huanwu decoction and Danshen decoction. Under high shear the whole blood viscosity is decreased distinctly by Shaofu Zhuyu decoction. After i.g. three pieces of prescription to stagnation blood stasis model rats, the erythrocyte deformability and the content of sialic acid of erythrocyte membrane is increased, SOD activity of erythrocyte membrane are increasing, the content of FIB is decreased significantly (P<0.05or P<0.01). The blood sedimentation K value could be decreased by Danshen decoction; Shaofu Zhuyu decoction not only could curtail APTT and decrease the content of MDA, but also could increase Na+-K+-ATP enzyme activity, improve depolymerization state of F-actin and increase the percentage composition of band1protein, band2protein and band3protein significantly.Conclusions:(1) The difference of three kinds of blood stasis model in macro-hemorheology registered as the following points:The cold stagnation blood stasis group demonstrated high rheological behavior; Starvation plus exhausted swimming group demonstrated low rheological behavior; Acoustics, optics, electricity complex stimulus plus clamp tail irritation group showed the curtailing time of APTT. In molecular rheology, SOD and Na+-K+-ATP enzyme activity of the three models were reduced, the content of sialic acid was decreased in the cold stagnation blood stasis group and the complex stimulus group.(2) The three prescriptions could improve the following performance in the cold stagnation blood stasis model rats such as whole blood viscosity and erythrocyte aggregation was increased, erythrocyte deformability was decreased and the content of FIB was increased; But Shaofu Zhuyu decoction was more effective than the other two in curtail APTT, increase Na+-K+-ATP enzyme activity and decrease the content of MDA; Most of all, Shaofu Zhuyu decoction had particularity in improve the abnormal phenomena of red cell membrane skeleton and band1protein, band2protein, band3protein.Danshen Yin influenced the band1and band7protein.