The Influence Of Sappanwood Ethyl Acetate Extract On NF-κB Of Heart Transplantation Model Rats And Apoptosis Of T-cell

Objective:Obverse the influence of Sappanwood ethyl acetate extract to NF-kB signal transduction pathway of heart transplantation acute rejection allogeneic rats and apoptosis of CTLL-2T-cells.Discuss the mechanism of the immune inhibition function of Sappanwood ethyl acetate extractMethod:Device32heart transplantation acute rejection allogeneic rats to four groups randomly which contain Cyclosporine A group, mix group(half Sappanwood and half Cyclosporine A), Sappanwood group(Sappanwood ethyl acetate extract), control group(model control group). Every group has8rats. Irrigate the rats'stomach seven days, then collect donor heart tissue and observe pathological change of donor heart with light microscope. Assay protein content of NF-kB P65in donor heart tissue by Western-blot method. Assay content of IkB-α mRNA in Real Time-PCR method. Foster CTLL-2of T cell externally, divide cells to high dose group,middle dose group,low dose group and blank group,after intervene with medicine4.5hours,test apoptosis of every cell group with flow cytometry detection technique.Result:(1)Compare with control group, Cyclosporine A group, mix group and Sappanwood group alleviate pathological damage of donor heart obviously and there are statistical differences between them (P<0.05). Compare with Sappanwood group, Cyclosporine A group, mix group alleviate pathological damage of donor heart obviously and there are statistical differences between them (P<0.05). The alleviate pathological damage effect of donor heart of mix group and Cyclosporine A group are fairly.There are no statistical differences between them (P>0.05).(2)Compare with control group, Cyclosporine A group, mix group and Sappanwood group restrain the express of NF-kB P65and IkB-a of donor heart obviously and there are statistical differences between them (P<0.05). Compare with Sappanwood group, Cyclosporine A group, mix group restrain the express of NF-kB P65and IkB-a of donor heart obviously and there are statistical differences between them (P<0.05) The restrain effect of the express of NF-κB P65and IκB-α of donor heart of mix group and Cyclosporine A group are fairly.There are no statistical differences between them (P>0.05).(3)Compare with control group, high dose group, middle dose group and low dose group all can promote T-lymphocytes apoptosis and there are statistical differences between them (P<0.05).Compare each other group, with the increase of dose,the effect increased.There are statistical differences between them (P<0.05)Conclusion:Sappanwood ethyl acetate extract can restrain the activation of NF-κB signal transduction pathway and promote apoptosis of external foster T-cell.All these maybe one of the mechanisms of the immune inhibition function of Sappanwood ethyl acetate extract...