Moxibustion C-26 Colon Tumor-bearing And Cyclophosphamide Chemotherapy In Mice Intestinal Mucosal Immune

Objective: This study is aimed to set up the murine model of mucosal immunodepression by in situ inoculating C26 colon carcinoma and repeated intragastric administration of cyclophosphamide. Detect some mucosal immunological index to understand the characteristics of these two models better. Then, we observe the effect of scarring moxibustion on the models, and explore its mechanism of mucosal immunology. At last, we compared me Th1/Th2 cytokine level in the blood and that in mucosa to understand the correlation between the mucosal immune and systemic immune.There are three parts in the study:1, Set up the mucosal immune deficiency models by in site inoculating C26 colon carcinoma with or without repeated intragastric administration of cyclophosphamide, and explore the its mucosal immunological characteristics.(1) Set up the mucosal immune deficiency models by in site inoculating C26 colon carcinoma with or without repeated intragastric administration of cyclophosphamide.(2) Study the mucosal immunological characteristics of the models.Ⅰ Establish a method to isolate lymphocytes from different parts of mucosal immune system.Ⅱ Explore the changes of T cell subsets composition in mucosal immune system between every group.Ⅲ Explore the changes of Thl/Th2 cytokines of mucosal immune system between every group.2, Study the effect of scarring moxibustion on mucosal immunologic function of tumor-bearing mouse with or without chemotherapy of cyclophosphamine.(1) Study the effect of scarring moxibustion on the T cell subset of different parts of mucosal immune system.(2) Study the effect of scarring moxibustion on the Th1/Th2 cytokine profile in mucosal immune system.3, Study the changes of Th1/Th2 cytokine level in blood in different groups. Materials and methodsFemale BALB/c mice were randomly allocated to sham operation group, tumor-bearing group, cyclophosphamide group, scarring moxibustion group and cyclophosphamid with scarring moxibustion group. 1×106 C26 colon carcinoma cells were injected into plasmamembrane of ceacum of each mouse to make the in situ colon carcinoma model, and the normal saline instead of colon carcinoma cells were injected to the mouse of sham operation group. After the inoculation the mice of cyclophosphamide group and cyclophosphamid with scarring moxibustion group were fed with 0.9% saline until the 12th day, from the 13th day to the 17th day the mice were treated with cyclophosphamide. The mice of scarring moxibustion group and cyclophosphamid with scarring moxibustion group were treated with scarring moxibustion for seventeen days after inoculation. All the mice were killed after the last treatment The area of Peyer's patch were measured and the morphological change of intestine of mice were observed. The T cells subsets of different parts of mucosal immune system including iIEL and LPL cells and Peyer's patch were separated and detected by immunofluorescence test and flow cytometer. The expression of IL-2, IL-10 in the local parts of intestine muscosal membrane were observed by immunofluorescence test. The amounts of IL-2 and IL-10 were detected by ELLISA. Results: 1 The establishment of mice model of C26 colon carcinoma with or without repeatedintragastric administration of cyclophosphamide and the exploration of the character of mucosalimmunity1.1 The results of the weight of tumor and the tumor inhibition ratio of cyclophosphamide suggested mat the tumor weight of tumor-bearing group was higher man cyclophosphamide group significantly, the tumor inhibition ratio of cyclophosphamide was 37.65% which confirmed that the establishment of mice model of C26 colon carcinoma with or without repeated intragastric administration of cyclophosphamide was successful.1.2 The area of Peyer's patch of sham operation group, tumor-bearing group, cyclophosphamide group mice were compared and the relativity of the tumor weight and area of Peyer's patch of tumor-bearing group mice was analyzed by statistical methods. We found mat the area of Peyer's patch of intestine in tumor-bearing group mice decrea...