Benefit Yangming Tianjin Gas Recipe Female Early Boss Rat Nervous Immune And Reproductive Axis Function In Rats

Objects: Through exploring the functional mechanism of herbs for"tonifying Fluid and Qi of Yang Ming" on Neuro-endocrine-immune system and H-P-O-A of the primary senile rat and in company with research in prophase and postphase. This research is to testify the importance of TCM traditional classic theory "Yang Ming Meridian Declines during Wu Qi period" in premenopause with prophase and countiue research. This research may offer a new study and differentiational thought to delay the aging of H-P-O-A during premenopause, improve premenopausal women health, prevent and treat relative diseases.Methods: 4⁶ months old SD female rats were set to be normal control group and 10-12 months old SD female rats whose oestrus elongation were set as primary senile female rat model. Primary senile female rats were devided into 5 groups randomly as following: 1. High dose Yiweitang group; 2. Middle dose Yiweitang group; 3. Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the blood samples from femoral artery.Then test the blood samples by the FCM (flow cytometer) to analysis content and proportion of CD₄⁺,CD₈⁺T Lymphocytes; by radio-immunity method. Ger the ovaries, hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method to.The rest hypothalami were made to homogenate, tested by radio-immunity method to detect β-EP, tested by fluorospectrophotometry to detect 5-HT, NE,DA.Results: In modulating H-P-O-A aspect, compound Yiweitang which could tonify YangMing Fluid and Qi could increase weight...