The Detection Of Intestinal Barrier Function And Intestinal Cellulose, L-glutamine On Intestinal Barrier Function

Aim: 1. To establish the method of measuring intestinal permeability using low dose of lactulose and mannitol in human. 2. To compare the difference of the intestinal permeability between the healthy volunteers and the postoperative patients.Method: Twenty healthy volunteer (Male : Female = 10 : 10, mean aged 36.7 ) and 20 patients of post laparotomy (Male : Female = 10 : 10, mean aged 52.7) were enrolled in the study. After an overnight fast, A 10 ml of test solution (containing 2 g of lactulose and 1 g of mannitol) was ingested. All urine for 6 hour from the time of ingestion was collected and the urine volume was recorded. Ten ml of urine with 2 mg of thimerosal was stored at -20 ℃ until analysis. After being centrifuged, desalted, deproteined and filtered, the sample was injected onto the column of HPLC with pulsed electrochemical detector for measuring concentration of lactulose and mannitol. Depending on the urine volume, dilution was needed. The urine excretion rate of lactulose (L%) and mannitol (M%) and their ratio (L%/M%) were calculated according to the ingested doseand urine volume. The difference of L % /M % between the healthyand the patients was compared using Student t test.Results: Two g of lactulose and 1 g of mannitol could be tolerated byall the subjects. No diarrhea was observed. Lactulose and mannitol...