Cucurbituril And Drugs And Biological Macromolecules Interaction And Analysis Applications

Proteins and nucleic acids are very important bio-macromolecules. Different proteins perform a wide variety of biological functions. Some proteins are enzymes, which catalyze chemical reactions. Other proteins play structural or mechanical roles, such as maintenance the metabolism of plants and animals directly express biologic characters. Nucleic acids play a central role in the storage replication of hereditary and aberrance information and in the expression of this information through synthesis. In order to surmount many difficult diseases and explore the secret of life, we further investigate the interaction between small molecules and nucleic acids or proteins. A rapid and convenient method is discovered for quantitatively analyzing nucleic acids and proteins.Based on the research of analytical chemistry and biochemistry, this dissertation investigated the interaction mechanism between small molecules, supermolecules or nanoparticles and proteins as well as small molecules and nucleic acids using the multi-techniques including fluorescence, resonance light scattering, absorption, Circular Dichroism, Transmission Electron Microscope and Scanning Electron Microscope. Several rapid, efficient methods, which are of high sensitivity, are developed for determining proteins and nucleic acids. This dissertation is written in six chapters.The progress of fluorescence probes and resonance light scattering probes for proteins is summarized. The progress of fluorescence probes for nucleic acids is recapitulated. The new macromolecule host compound cucurbituril (CB) and the interaction between CB and metal ions or small organic molecules were reviewed in the first chapter. References (188) are cited here.Second chapter describes the properties and the scope of application of CB. It is found that CB can enhance the fluorescence of 1,8-ANS-BSA system in...