Polygonum Cuspidatum Resources, Quality And Efficacy Study

Huzhang, Rhizoma et Radix Polygoni Cuspidati, was widely used in China for a long time. According to the investigation on the resources of Polygonum cuspidatum, it was discovered that the Polygonaceae plant, Polygonum cuspidatum Sieb. Et Zucc. distributed in 23 provinces in China totally. However, the resources had been severely damaged in these places by degrees. The original productive place was not in Fenzhou as ancient documents recorded, but was in Southwest China and south Shanxi province. Therefore, the distribution of the resources should be districtly optimized for continuous utilization. We suggest to set up some planting bases of Huzhangi with high quality on these areas.After researching on the active components of Huzhang in different productive areasand different seasons, we found that the contents active components in phloem was higher than in xylem. The active components accumulated most in September in plants. Compared with the standard of Huzhang in Chinese Pharmacopoeia, the content of emodin in Huzhang from part of the production areas or from market was slightly decreased, while the content of polydatin was significantly 10-15 times more than that of the standard. Meanwhile, contents of heavy metals in Huzhang accorded with limitation standard as Chinese administration declared. As a good result, it will benefit for exportion of resveratol, which was extracted from Polygonum cuspidatum. The HPLC fingerprints of Polygonum cuspidatum was established for the first time. Results indicated that the HPLC fingerprints of Huzhang from different productive places were similar.RAPD, ISSR, SRAP marker techniques were applicated to study the genetic diversity of germplasm resources of Polygonum cuspidatum and its DNA fingerprints was established. With RAPD, ISSR, SRAP marker, the DNA fingerprints of Ploygonum cuspidatum were established for the first time. The germplasm resources of Polygonum cuspidatum has a rich genetic diversity. We harvested 98 DNA fragments with 22 primers. 90.8% of the DNA fragments had genetic diversity, while products from 17 primers of the total 22 primers had genetic diversity. Based on cluster analyzing, we found that the genetic diversity of germplasm resources of Polygonum cuspidatum were related to its geographic distribution. For the first time the endogeny fungal populations in Polygonum cuspidatum were studied. Results show that the fungals taxa and quantity showed varieties. The accumulation of two components...