Experimental Study Of The Hepatitis B Virus Hbsag Dna Vaccine

Hepatitis B is one of the most popular disease in China which is still lacking effective therapy. HBsAg vaccine can provide protective immunity and reduce incidence of the disease remarkably after vaccination in the new-bombaby. HBsAg vaccination is the so far the most effective and economic method to control the infection and epidemic.Currently used HBsAg vaccine is recombinant vaccine pruduced by genetic engineering. It provides protective immunity up to 90% when vaccinated in people. But there are still some disadvantages, such as ineffectiveness in about 10% of people vaccinated, slow induction of antibody, and lack of cellular immune response. So, new kinds of vaccine which have better effect are needed. DNA vaccine is a good candidate. Because it can induce not only specific antibodies, but also specific cytotoxic T lymphocytes in animal experiments.The open-reading-frame of S in HBV genome can be translated to 3 kinds of proteins, s protein is the main component of the engineered vaccine, which carrys most of the immunologic epitopes. Pre-s1 and pre-s2 proteins can induce immunity independent to s protein. Immunity induced by them is controlled by different sites of MHC gene. Th lymphocyte induced by pre-s1 and pre-s2 can also assist in production of antibody to s protein. In our study, S gene was selected to construct recombinant DNA vaccines.pcDNA3.1(+) and pAdCMV vector both have CMV promoter and SV40...