Phlegm And Blood Stasis And The Therapeutic Effects And Mechanisms Of Polycystic Ovary Syndrome Rats

Polycystic ovary syndrome(PCOS)is one of the most common complicated syndrome for child-bearing age woman,which is caused by endocrinopathy and sugar metabolic anomalies and featured by higher androgen level,no- ovulating for a long time,obese, pilosity,abnormal menstruation,sterile.The incidence rate is 5-10%in the woman of child-bearing age,and 50-70%in sterility women having no-ovulating.It becomes the most popular researching topic in gynecology field for its biochemistry characteristic and the clinical manifestation and pathogenesis's non-uniformity.The clinical epidemiology research also identically discovered that the occurrence probability of diabetesⅡ,hypertension,cardiac disease,hyperlipemia,endometrial cancer,hypertension of pregnancy syndrome and pregnancy diabetes for PCOS patients increases obviously.Therefore,the correction to the symptoms caused by excessive androgen,such as obese,the pilosity,the acne, abnormal menstruation,sterile and so on,and the prevention from every kind of near future and the forward complication are both terribly critical to woman's life and health as well as living quality.The traditional Chinese medicine believes that the kidney storing the essence and dominating reproduction,the deficiency of kidneys vitalness is the foundation of sickness,and is signed by phlegm,the ecchymosis hinders,in the treatment to invigorate the kidney the adjustment internal organs function primarily,the sign reality in effects a permanent cure in the foundation,either removes extravasted blood or the phlegm reduction.Therefore,to study out the traditional Chinese medicine compound prescription for kidney-invigoration,phlegm reduction,extravasted blood-removal.And to explore its influence to reproduction endocrine system and the sugar metabolism as well as the influence to Leptin,Adiponectin mRNA expression by the the Poresky law inducing the multi-pouch ovary syndrome animal model,and to research compound prescription traditional Chinese medicine drug efficacy and the possible function mechanism.PartⅠPre-experiment for the PCOS animal model induced by Poresky MethodThe pre-experiment with Poresky Method inducing PCOS animal model,observe the influence of the Chinese medicine compound prescription intervene to rats ordinary circumstances,the body weight,the vagina cell smear.In the mean while,to study the changes of serum sex-hormone,FPG and FINS.The result indicated:The PCOS animal model of Chinese medicine deficiency in kidney and phlegm stasis could be successfully worked out by the Poresky method.The rats body weight increases by the model group obviously,the traditional Chinese medicine group and the model group are compared,the difference have the significance, P＜0.05.Blood Sex-hormone level comparison:T and the LH level,is highest by the model group,traditional Chinese medicine group next best.The traditional Chinese medicine group and the model group compare,have the remarkable difference,P＜0.05.The LH/FSH ratio is highest by the model group,the traditional Chinese medicine group and the model group compare,the difference does not have the significance,P＞0.05,but compares the model group to assume the obvious declining trend,the FBG level,take model group as highest, the traditional Chinese medicine group and the model group compare, the difference does not have the significance,P＞0.05,but the value compares the model group to reduce;Rats FINS level,take model group as highest,the traditional Chinese medicine group and the model group compare,P Has the extremely remarkable difference.The rats vagina cell smear change model group majority does not have the ovulation;the traditional Chinese medicine group part has the ovulation.PartⅡThe influence of compound prescription traditional Chinese medicine to PCOS model rats serum sex hormoneThis experiment to makes the mold by Poresky law,observation to the rats's ordinary circumstances,then to appraise the influence to serum sex hormone T,FSH,and LH after treatment with traditional Chinese medicine,western medicine (Rosiglitazone),union medication respectively.The experimental study result proved that the traditional Chinese medicine group,Rosiglitazone group,and the union medication group all could decrease the ratio of serum testosterone level,the LH level,the LH/FSH,what's more,among the three groups, the unite the medication group is the best and better than surpasses Rosiglitazone group;the the union medication group is the most obvious for the serum LH level;At the same time,the union medication group can also obviously improve the LH/FSH ratio situation;Regarding the body weight change's research,the traditional Chinese medicine group and and the union medication group are both effective to lose weight,but the union medication group is better Rosiglitazone group.PartⅢThe observation to PCOS model rats ovary teetologyIn the interest of evaluation model's success and pharmacological treatment's effect,further observed the ovary tisuess pathology HE dyes of each group ofthe rats.Through the observation the change of ovary gross anatomy and the graafian follicle shape,We have an objective appraisal to the PCOS model, in the mean while,observed the change of the ovary tisuess pathology after treatment.The research proved:the ovary weight after treatment, traditional Chinese medicine group and the model group compared, have the remarkable difference,P＜0.01.It means that the ovary weight and the ovary average area have the obvious drop with the compound prescription traditional Chinese medicine treatment.The pathological section is the objective indicator to measure ovary morphological change,after the compound prescription traditional Chinese medicine treatment,the histo-pathology inspection discovered only little antrum follicles in PCOS rats ovary,follicle cavity is not big,and the follicle wall granular cellular layer and corpus luteum(yellow body)quantity increase obviously compared with that in model group,which proved that PCO have been improved and ovulation function restorated after the traditional Chinese medicine treatment.PartⅣThe influence of compound prescription traditional Chinese medicine to PCOS model rats sugar metabolismThis experiment to makes the mold by Poresky law,to treat respectively by traditional Chinese medicine,western medicine (Rosiglitazone),union medication,observation the rat's weight and blood sugar changes after treatment,then to appraise the influence to serum INS,IGF-1.to explore the influence of compound prescription traditional Chinese medicine to PCOS model rats sugar metabolismThe result demonstrated that:the PCOS model rat's weight have been decreased in the compound prescription traditional Chinese medicine group,union medication group,and the union medication group surpasses Rosiglitazone group.FINS level,FPG level,insulin (Homa-IR)of the model group is obviously higher than the normal group,which proved that the insulin resistan model for PCOS rats is successful.After the treatment,each group can decrease the serum INS level,but the effect of union medication group to reduce serum INS surpasses that of the compound prescription traditional Chinese medicine group;After the treatment,the union medication group and Rosiglitazone group can decrease the FPG level effectively,but it's not so effective for the traditional Chinese medicine group. After the treatment,each group drops with insulin resistance index (Homa-IR),but the union medication group and Rosiglitazone's function surpasses the compound prescription traditional Chinese medicine group,especially the union medication group is the best. Regarding the comparison of blood sugar curve under the area,model group is the highest,other groups drops after treatment,of which the union medication group drops most.Model group serum IGF-1 level is much higher than the compared group obviously.All the three kinds of treatment can reduce serum IGF-1 Level,of which the union medication group is better compared with the compound prescription traditional Chinese medicinegroup.PartⅤThe analysis of PCOS model rats serum Leptin level and correlation factorThis experiment to makes the mold by Poresky law,to treat respectively by traditional Chinese medicine,western medicine (Rosiglitazone),union medication,to observate the rats's serum Leptin level changes after treatment,explore the influence of compound prescription traditional Chinese medicine to PCOS model rats and possible function mechanism by the analysis of PCOS model rats serum Leptin level and correlation factor(FSH,LH,LH/FSH,T,IGF-1,INS,Weight)The research discovered:Model group's Leptin level is the highest among groups,other groups all reduced.The normal group and the model group compared,the difference has the significance, P＜0.05,the model group serum Leptin level is obviously higher than the normal group,which is similar with previous research results. After the treatment,although Rosiglitazone group and the union medication group can lower the Leptin level obviously,and the traditional Chinese medicine group is not obvious,it's obviously lower than the model group in the value,the traditional Chinese medicine function waits for further confirmation with the increase of sample quantity.Through the analysis of the rats Leptin and FSH,LH,LH/FSH,T, IGF-1,INS,the Weight,it's discovered that the relationship between the rats's Leptin and LH,T,IGF-1,INS have been positively, and have been no relationship with other indexes.Through the analysis of T and LH,INS,the Weight,it's discovered that T and LH,INS,Weight related positively.PartⅥThe research of PCOS model rats serum APN determination and fatty tissue APN-mRNA expressionThis experiment to makes the mold by Poresky law,to treat respectively by traditional Chinese medicine,western medicine (Rosiglitazone),union medication,after the measurment to the rats serum APN level's change by ELISA method,explore the influence and the possible function mechanism of compound prescription traditional Chinese medicine to the PCOS model rats.The research discovered:Model group's serum Adiponectin level is the lowest compared with other grops,,other groups all increase. The normal group and the union medication group and the model group compared,the difference has the remarkable significance,P＜0.01. The traditional Chinese medicine group and Rosiglitazone group and the model group compared,difference obviously,P＜0.05.The traditional Chinese medicine group and Rosiglitazone group and the union medication group compared,the difference have the significance,even P＜0.05.The traditional Chinese medicine group and Rosiglitazone group compares,the difference does not have the significance,P＞0.05.It proved that three methods can all elevate the serum Adiponectin level,but the union medication group is the best,compared with the traditional Chinese medicine group and Rosiglitazone group.Expression of Adiponectin mRNA in fatty tissue of each group, the model group is the lowest,other each group elevates.And the normal group and the model group compared,the difference has the remarkable significance,P＜0.01.The union medication group and Rosiglitazone group and the model group compared,the difference has the remarkable significance.P＜0.01.The traditional Chinese medicine group and the model group compare,the difference has the significance.P＜0.05.Comparisons of the traditional Chinese medicine group and Rosiglitazone group,the union medication group three groups,the difference does not have the significance.P＞0.05.Explained that three methods can increase Adiponectin expression in fatty tissue,and the difference between each other does not have the significance.