Study On Synthesis Methods Of Ginseng Sulfated Polysaccharides And Multiresidue Analysis Methods Of Organochlorine Pesticide

Panax ginseng C.A.Mey. is a plant of Araliaceae. Ginseng with high reputation and expensive value is usurally regarded as one kind of Chinese traditional herb which has many medical effecets, such as keeping the halth in balance, making the blood more fresh, keeping the cough off earlier, and delaying the life period, etc. Extracted from panax ginseng, Polysaccharide, as one of the main active components, has various fuctions such as anti-tumor, anti-caducity, decreasing blood sugar and blood fat, and modulating immnity, etc.The thesis introduces the Ginseng polysaccharides which are extracted from fresh root of ginseng and belongs to one kind of D-dextrans. A kind of sulfated polysaccharides was obtained and its struction was proved. A new method for analysis of nineteen organochlorine pesticide residues in Ginseng is proposed.1. The best conditions and methods of extracting Ginseng polysaccharides are indentified through comparing the water extraction-ethanol deposition. The average molecular weight is about 10,300 of Ginseng polysaccharides by HPLC. The molecular structure is Pyran-typedα-D- dextrans by FT-IR.2. Ginseng sulfated polysccharides was obtained from Ginseng polysaccharides with chlorosulfonic acid-pyridine in this paper first. The best conditions of synthesis through comparing the effects of different conditions such as vol.proportion of chlorosulfonic acid-pyridine, reactive temperature and reactive time. Replace degree of S is 2.05.3. By means of HPLC, UV and FT-IR, the structure of ginseng sulfated polysccharides was proved. The HPLC chromatogram shows a single peak; the UV spectrum of Ginseng sulfated polysccharides shows the characteristic absorptions of sulfate ester bond at 250～275nm; the FT-IR spectrum of Ginseng sulfated polysccharides shows the characteristic absorptions of sulfate ester bond at 1257cm^-1 and 826cm^-1. These show that synthesis of the sulfate ester is made.4. The ¹³C NMR spectrum of Ginseng sulfated polysccharides shows that hydroxyl of C-6 is more active than those of C-2, C-3 and C-4.5. The multiresidue analysis method of nineteen organochlorine pesticide were established which qualifies the precision and accuracy corresponds with requirement of the analysis of the pesticide residues. The good linearity were obtained for nineteen organochlorine pesticide with the average relative coefficient larger than 0.992. The minim detectable sample was1.5×10^-13～2.5×10^-12 g, and the average recoveries range was from 75.7% to 115.2%.6. In the thesis, Ginseng sulfated polysccharides synthesized include some characteristics of homogeneous component, lesser molecular weight, more replace degree of S, which provide the sample for the more biology-action of anti-AIDS and anti-tumour.7. The multiresidue analysis method in the thesis may be applied in some fields widespreadly and provides good technoloy of supporting gensing quality improvement to increase the competitive capacity of International ginseng market, accelerate the development and modernazation of the profession of gensing with the advanced means of testing.