Effect Observation On Rheumatoid Arthritis And Intervention Effect On Synoviocytes Cells Of Biqing-drink

This study makes further exploration of the pathology mechanism of active rheumatoid arthritis(RA) in theory, clinical and experimental tripartite face with theories of TCM and Western Medicine Combined. The theories of"heat-toxicant etiology"and"heat-toxicant causing rheumatism doctrine"are advanced under the guidance of professor Zhang Minghe and my tutor professor Zhou Cuiying.We think that main pathology mechanism of RA in periods of activity is damp-heat and stasis -toxicant obstructing channels and flowing to limbs'joints. The key treatment methods are clearing away heat and toxic materials, clearing away dampness to remove obstruction in the channels and promoting blood circulation to remove blood stasis. 60 patients were divided into two groups(test group 30 patients and control group 30 patients).The patients in test group were treated by Biqing-drink decoction and Methotrexate, those in control group were treated by Pafulin(Radix Paeoniae Alba glycosides ) . The standards of curative effect are ACR20,ACR50 and ACR70 made by rheumatism institute of U.S.A. Results: the test group ACR20, ACR50, ACR70 efficiency were higher than the controls, but the difference between the two groups was not significant (P> 0.05); Syndrome effect of Chinese medicine, the total efficiency of the two groups no significant difference ( P> 0.05), the rate charged was more significant difference (P <0.05), Syndrome effect in controlling the rate of the test group than the control group were to improve the clinical symptoms and signs, laboratory and other aspects of effective indicators, The test group than the control group (P <0.01, or P <0.05); test group and control group has a good safety, between the two groups was not significant (P> 0.05); test group and has a good tolerance , Compared with the control group had a significant (P <0.05). Experimental study aimed to investigate RA fibroblast-like synoviocytes (fibroblast-like synoviocytes, FLS) cell growth characteristics, the application of MTT, flow cytometry and RT-PCR, and other methods to detect different concentrations of the Biqing-drink on RA FLS -proliferation and differentiation, and control of TGP.The results showed that: Both Biqing-drink and TGP can inhibit the proliferation of FLS; Biqing-drink can inhibit the FLS mitosis, shortening S phase, to extend G1 phase; Biqing-drink increase FLS bax mRNA expression, lower FLS bcl-2 mRNA expression , suggesting that the Biqing-drink directly inhibit cell proliferation in the transcription level, and the role of TGP similar. Conclusion: "Toxic Heat causes theory" and "Toxic Heat to Bi" for the incidence of active RA and the outcome of major aspects of development, the efficacy of Biqing-drink treats active RA may in the anti-infective, anti-inflammatory, analgesic, inhibit immune inflammatory response, regulate immune function and improve the mechanism of coagulation and fibrinolysis;Its mechanism is that Biqing-drink can inhibit the proliferation and differentiation of RA-FLS.