Changes Of The Voltage-depended Calcium Channels In The Human Ejaculatory Sperm Of The Asthenospermia

Purpose : To explore the relationship between the changes of the voltage-dependent calcium channels functional in the asthenospermia sperm and asthenospermiaMethods: Sperms were aseptically obtained by masturbation and prepared by swim-up technique to produce a spermatozoa solution of high motility from 20 healthy fertile men. The spermatozoa solution was incubated with the different types CCBs at 37℃in vitro. Motive parameters were measured by computer-aided sperm analysis (CASA). Measurements were carried out at 10,20,30 and 60 minutes in all specimens included live rate, progressive motility, malformed rate, VAP, ALH and WOB. then use reverse transcription polymerase chain reaction to examine and quantitate the multitype VDCCsα1 mRNA expressions between the normal and the asthenospermia. The last to use Real-time qPCR, Western blotting and Immunocytochemical technique to examine the changes of L type VDCCα1C between the normal and the asthenospermia.Results: The results showed that the nifedipine can not only significantly affect human sperm's shape but also spermatozoa motility incubated in vitro (p<0.001). Comparing with the normal sperms, there are the markedly differences in the expressions of the asthenospermia'sα1B,α1C,α1E,α1G andα1H mRNA messages(p<0.01). and there are the markedly differences of the asthenospermia's L type VDCCα1C at molecular level, protein level and cellular level.Conclusions:L-type CCB can directly cause reversible male infertility in vitro. Based on VDCC mRNAs relative expression levels, we propose that both L-type and non-L-type VDCCs all may be gates for the external calcium influx, required for the motility. Changes of the L type VDCCα1C gene influenced motility of normal sperm significantly, and it would be a new target of the study and treatment in asthenospermia.