| Gastric ulcer(GU) is one of the common diseases in clinics, processing the characteristics of long course, recurrence, complex performance. The etiology and mechanism of onset of GU have not been fully clarified yet in modern medicine. The treatment of dradication of helicobacter pylori and anti-acid can get good efficacy, but partful verrucous lesions often persist and do not dissipate.The recurrence rate of the ulcer conventionally treated with anti-acid one year after the withdrawal can be as high as 60%. Professor Zhou Xuewen create the etiological theory of toxin-heat, according to the clinical manesfestations and the morphological features of gastroscope of GU. The research work is to discuss the efficacy and pharmacodynamics mechanism of the decoction Xiaoyongkuidekang(XYKDK) composed according the theoraputic method of clearing away the heat while detoxifying the body, and elimianting the carbuncle while growing muscle, so as to discuss the mechanism of treating gastric ulcer with TCM. The pathogenisis of toxin-heat and theoraputic thinking of treating peptic ulcer as carbuncle are theoretically expounded.ObjectsThis study estimated the acetic acid-induced gastric ulcer in rats treated with XYKDK by immunohistochemical staining and computer image analysis quantitative assessment.It also investigated the possible therapeutic mechanisms of XYKDK treating gastric ulcer from the aspect of growth factor and trefoil factor, so as to further explain the eitology of toxin-heat and the acdemical thinking of treating peptic ulcer as carbuncleMethods1. The rat model of acetic acid-induced gastric ulcer was established. And the rats were devided into six groups: blank control, GU model, Weikang capsule, Cimetidine, XYKDKâ… (small dose) and XYKDKâ…¡(1arge dose)groups.2. To treat the rat model according to the research plan.3. The normal tests were conducted after treatment, including measurement of gastric juice, gastric acidity, ulcers area, as well as the observation of stained sections of ulcer tissue.4. Gastric ulcer index and gastric ulcer inhibition ratio were measured, and the condition of ulcer healing was evaluated.5. The content of epidermal growth factor (EGF) and the expression of its receptor(EGFR) were measured to evaluate the efficacy by the radioimmunoassay method.6. The expression of trefoil factor(TFF)was determined by molecular biology techniques.Results:1. XYKDK has effecet on healing factors of gastric ulcer in rats, reducing gastric juice, gastric acidity, the damage of the gastric mucosa and ulcers area, and promoting healing of the ulcer, which have Dose-effect relationship.2. XYKDK promotes the formation of ulcer granulation tissue, increasing regenerated mucosa thickness, reducing the ulcer index significantly, which have Dose-effect relationship.3. XYKDK improves the EGF concentration in rat gastric ulcer tissue, increasing the EGFR expression in mucosal ulceration, enhancing regeneration and repair capability of mucosa, so as to accelerate ulcer healing.4. XYKDK increases the expression of TFF in rat gastric ulcer tissue, enhancing regeneration and repair capability of mucosa, so as to accelerate ulcer healing.Conclusion:The theorapetic method of clearing away the heat while detoxifying the body, and elimianting the carbuncle while growing muscle is effictive, and the etiology of toxin-heat do exist during the onset of gastric ulcer.
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