Building Of Central Anorexia Animal Model And Study Of Therapeutic Action Of Decoction Of Jian Pi Xiao Shi In Treating Anorexia

Backgrounds and aimsInvigorating spleen Chinese medicines to promote digestion are of much more features in the whole of Chinese medicines,those Chinese medicines can obtain some good effects in treating such diseases as abdominal,belch,bepanthen,nausea and vomiting, anorexia,diarrhoea,adstricto,etc,resulting from fresh plot stagnation.At present, pharmacological researchs for those kinds of Chinese medicine almost began with studying the drug effect in stomach intestine & function,there is few literatures about pharmacological research for Chinese medicine in respect of adjusting central nervous system at home.We intend to take apositia as the object of study,which is a disease of losing appetite frequently occurred.At present,most of studies of apositia began at first to adopt animal model of splenic asthenia,there are many patterns of syndrome of apositia in the clinical practices,the model of splenic asthenia cannot represent all the models of apositia.The center of adjusting the appetite in the body activities lies in the nervous system,the root cause of apositia is controlling disorder of the central nervous system,this sdudy intends to establish an animal model of apositia with the function of central inhibition according to the cause of disease to give some reference for pharmacological research of apositia.Shanzha Maiya granula is the product of Zhongshan Zhongzhi Medical Industrial Group Coporation Ltd,mainly composed of two kinds of medicines promoting digestion and invigorating the spleen,with good effect in the clinical trial,this Laboratory's pharmacodynamic research also illustrates that this product can play an important role in promoting stomach intestine and increasing the animal's weight & food intake.Decoction of Xiangshasix Mild Drugs is a classical prescription of long history,which has good effect in treating the loss of appetite arising from Qi stagnant or sputum stasis.This topic intends to set up the animal model of apositia with the function of central inhibition to investigate the pharmacological effect of Shanzha Maiya granula and research the functional mechanism of the Chinese medicines for invigorating spleen to promote digestion in view of braingut petide-appestat.Methods of research1.screening some methods of setting up the animal model for children's loss of appetite,including feeding the young mouse with the feeder of high nutrition made of eggs, milk,pork,sugar,etc;intraperitoneal injection of Cholecystokinin(CCK);intraperitoneal combined injection of Cholecystokinin(CCK) and Leptin(LP);pre-setting canula to inject LP in the lateral cerebral ventricle,etc.2.on the basis of successfully establishing the animal model of apositia,concentrates on investigating the model's effect on the mouse's whole pattern,weight and food intake, detecting the content of neuropeptide Y in the mouse's hypothalamus & blood plasm,which plays key role in eating,and mastering the medicine's effect on the mouse's braingut petide-appestat after setting up the animal model.Investigate the medical effect of the water extract of Decoction of Xiangsha six Mild Drugs and Shanzha Maiya granula on the model mouse,and detect the content of NPY in hypothalamus & blood plasm.3.set up the indicators of qualitatively and quantitatively detecting Shanzha Maiya granula,and investigate the pharmacodynamics indicator of the Shanzha Maiya granula's effect on the animal's stomach intestine & food-intake,etc.Results1.the model method of feeding the young mouse with the feeder of hyperalimentation may make the mouse to eat less,and make the mouse' weight to increase slowly, however,after stopping the model method and returning to feed the mouse normally,the mouse' weight & food-intake increase quickly.During the time of making the model,the cause of the mouse's apositia is the mouse disliking those foods composed of high-fat & high-protein substances,not loss of appetite;none of the 3 groups of intraperitoneal injection of 5μg/kg,10μg/kg,20μg/kg Cholecystokinin(CCK) can make the mouse's appetite to lower;the intraperitoneal combined injection of Cholecystokinin(CCK) and Leptin(LP) cannot make the animal's appetite to lower,it is likely that the medicine's failure to inhibit the appetite results from the animal's bad absorption or the time of the medicine taking effect being too short,etc.2.establishing successfully the animal mode of apositia through injecting Leptin (LP).This method can be illustrated as follows:at first,set canula in the lateral cerebral ventricle of mouse(take the mouse weighting about 300 grams as example,it is fit to bury the canula needle in 1.5cm after anterior fontanel,1.5cm left or right,4ram deep);after the mouse's recovery for a week,inject in the every day the mouse with the dose of 10μg/20μl/kg leptin at speed of about 1 macro-litre as per minute.After making the model, the mouse eats about 13 gram of feeder everyday,its weight reduces steadily about 6-10 gram everyday on the average.After the termination of the model,kill the mouse,detect NPY and then see the content of NPY in the Hypothalamus of the mouse significantly lower.3.8g/kg(it is valid dose proved by pharmacodynamic experiment,about 9 times of human dose) of Shanzha Maiya granula and 13.6g/kg(15 times of human dose) of Decoction of Xiangsha six Mild Drugs can play a certain role in treating the animal model of child apositia resulting from leptin,fail to increase the animal's food-intake,but can increase its weight to some extent,Decoction of Xiangsha six Mild Drugs can increase the content of NPY in the Hypothalamus of the mouse.4.set up quality standard of qualitatively and quantitatively detecting Shanzha Maiya granula:detecting the content of aurantiamarin as a main component of Chenpi in Shanzha Maiya granula through the method of high performance liquid phase,qualitatively identifying Shanzha,Maiya,Chenpi through the method of plate chromatography.The pharmacodynamic experiments illustrate that 8g/kg of Shanzha Maiya granula's effect is the best in promoting stomach intestine,increasing food-intake and weight.ConclusionThe mouse model of intraperitoneal injection of leptin causing apositia which makes the mouse's appetite & weight to lower,can inhibit expression of NPY in hypothalamus, conform to the principle about mechanism of body appetite's adjustment,so the principle of the model causing apositia is similar with the incidence mechanism of apositia,Shanzha Maiya granula and Decoction of Xiangsha six Mild Drugs as Chinese medicines for invigorating spleen to promote digestion can play an important role in treating the model mouse,the functional mechanism of Decoction of Xiangsha six Mild Drugs is related to adjustment of NPY in hypothalamus,so this model which is used to researching the mechanism of treating child apositia,also may be used as pharmacological model fit for other Chinese medicines for invigorating spleen to promote Qi.