| Objective:To observe the effect of huanglianwendan decoction onmetabolic syndrome food-inducing rats,and Start with adipocyto-kines andendodermis function to discuss the molecular mechanism thathuanglianwendan decoction prevents and treats MS.Method:Sixty 8-week-old male SD rats were randomly divided into 2groups which are vacuity group (n=10)and experimental group (n=50)vacuity group was fed with common diet,and experimental group was fedwith high lipid,high salt and high glucose diet for 20 weeks to induceexperimental metabolic syndrome.37 SD rats in experimental group whichwere modeled successfully were randomly divided into 4 groups:modelgroup huanglianwendan decoction high-dose group,low-dose group andMetformin Hydrochioride control group,which were lavaged with theappropriate drug for 4 weeks.Dynamic state measure weight,blood pressure,fasting blood glucose,serum insulin,lipids and other indicators,calculateHOMA-IR,to evaluate model and drug effect.Before and after drug therapy,we observed the pathological change of aorta by HE staining method,andtested:the vWF factor levels of serum by ELISA method;the level of FFA,LEP,TNF-α,IL-6 and ADPN in serum by RIA method;the expressions ofADPNmRNA in adipose tissue by RT-PCR method in each group.Result:After feeding for 20 weeks,the weight,abdominalcircumference,Lee's index,blood pressure,fasting glucose,fasting insulin,HOMA-IR,lipids TG,TC and LDL of Experimental rats were significantlyhigher,HDL level of reduction,compared with the vacuity group,thedifference was significant (P＜0.01).After 4 weeks treated with drugs,compared with the model group,theweight and blood pressure decreased in huanglianwendan decoction high andlow-dose group and the metformin group,in which huanglianwendandecoction high-dose group and metformin group weight loss significantly (P＜0.01).The blood pressure in Huanglianwendan Decoction high-dose groupdecreased obviously,compared with the model group,it was statisticallysignificant (P＜0.05).the blood glucose of huanglianwendan decoctionhigh-doses group and metformin group decreased with varying degrees (P＜0.01),(P＜0.05)Compared with the model group,there were remarkabledifferentce In the TG,TC and LDL-C level of huanglianwendan decoctionhigh doses group P＜0.01).The serum insulin levels of huanglianwendan decoction high-dose group and metformin group were lower than those inmodel group (P＜0.01),(P＜0.05).The insulin resistance index(HOMA-IR)in huanglianwendan decoction high-dose group and the metformin groupdecreased,and there was significantly difference (P＜0.01).The Lee's index,visceral fat weight,visceral fat and body weight ratio in huanglianwendandecoction high-dose group and the metformin group were significantlydecreased,the difference was remarkable (P＜0.01).The MS model group rats had serious endothelial injury in aortic Hstaining;huanglianwendan decoction high-dose group,low-dose group andmetformin group,there are amelioration in endothelial injury with varyingdegrees;the huanglianwendan decoction high-dose group had the mostobvious amelioration.Compared with the vacuity group,the serum vWFlevels of MS model group were significantly higher (P＜0.01),comparedwith the MS model,the vWF level in huanglianwendan decoction high andlow dose group were lower significantly (P＜0.01).Serum FFA,leptin,TNF-αand IL-6 concentration in MS model groupincreased,Compared with the vacuity group,the difference was obvious (P＜0.01);compared with the model group,the FFA level in the other threegroup decreased remarkably.serum leptin,TNF-α,IL-6 concentrations ofhuanglianwendan decoction high-dose group and metformin group ratsdecreased (P＜0.01).Compared with the vacuity group,the ADPN level landrelative quantitative gene expression of ADPNmRNA in model groupsignificantly reduced (P＜0.01);compared with the model group,the ADPNlevel of huanglianwendan decoction high-dose group and metformin groupincreased (P＜0.05);the relative quantitative gene expression of ADPNmRNA in huanglianwendan decoction high-dose group increased,(P＜0.01).the relative quantitative gene expression of ADPN mRNA in Metformingroup had no significant difference (P＞0.05).Conclusion:1.High fat high sugar high salt diet feeding SD rats forLong term can copy the MS model with the basic human clinical features ofMS.2.Huanglianwendan Decoction can loss weight,lower blood pressure,regulate lipid and improve insulin resistance,which can improve the variouscomponents of MS.The comprehensive effect of huanglianwendan decoctionis superior than that of metformin.Huanglianwendan decoction is aneffective drug on preventing and treating MS,which has the value to researchand develope further. 3.there is endothelial dysfunction in MS rats,but huanglianwendandecoction can reduce the level of serum vWF and improve the organizationalstructure of the MS rat aorta to improve vascular endothelial dysfunction.4.Huanglianwendan decoction has the effect on reducing serum TNF-α,IL-6 levels of MS rats and inhibiting inflammatory response.5.Huanglianwendan decoction can reduce the levels of FFA,leptin andadiponectin in the MS rats serum and promote the expression of adiponectinmRNA,which has obvious effect of regulating adipocytokines.6.Huanglianwendan decoction can regulate the expression ofadipocyto-kines and inhibit inflammatory response,which may be one of themechanisms to improve vascular endothelial function in MS. |
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