| objectiveThe Prescription of Patecy Lung and preventing asthma,which is used in clinical medicine is preliminary exploration in immunization for many years,and effect was Significantly. Inhibition of value-added for smooth muscle cell of bronchus were studied though Serum pharmacology method in-vitro method assay; Thought in-vivo experiment,the affect of preventing asthma and lung morphology to the model asthma pig mouse which were medicated by this Prescription were observed.The diversification of EOS,IgE,Eotaxin of correlation factor and expression level changes of adhesion molecule ICAM-1 in blood and the bronchoalveolar lavage fluid.Asthma mechanism of prescription of Patecy Lung and preventing asthma was probed through cell and molecular biology level,and this also gave a foundation for the development clinical of traditional Chinese compound prescription to prevent asthma.Methods1,In vivo experimental:first,cells of big mouse were differential counted,then contents of Eotaxin and ICAM-1 of supernatant fluid of cells were surveyed and evaluated by ELISA.Contents of IgE were detected by dfferential blood count and radioimmunoassay. Prepared tissue slice of Lung and bronchus from big mouse,and stained with Hematoxylin Eosin(HE),then Observed gas channel restructuring situation;Gene expression of Eotaxin and ICAM-1 from Lung and bronchial tube partial organization were investigated by immunohistochemistry.2,In vitro method:Including medicine blood serum was prepared;Proliferation level of Smooth muscle cell from bronchus were tested by MTT enzyme label ling and ICC method.Results1,In vivo experimental:1.1 The results were as follows: respiratory tract inflammation symptoms were improved obviously by prescription of Patecy Lung and preventing asthma,because quantity of WBC especially acidophilic leukocyte were cut down significantly decreased(P<0.01).It can also show that treatment and prevention function was realization by reduced the content of blood serum IgE.Those can give the Powerful experiment basis of Clinical practice against Bronchial tube asthma for prescription of Patecy Lung and preventing asthma.1.2 ELISA demonstration:the earlier period research indicated that the EOS was obviously controlled by prescription of Patecy Lung and preventing asthma, because the contents of Eotaxin and ICAM-1 of bronchia alveolus lavage fluid of control group were reduced obviously compared with that of normal group after Treatment.Remarkable pathology physiology characteristic of asthma inflammation was EOS infiltration.EOS hastened and collected to the inflammation spot were important pathology process in asthma illness,which were closely related to Eotaxin and ICAM-1.These results suggested that inhibitory action of Eotaxin and ICAM-1 possibly was as asthma's mechanism function of prescription of Patecy Lung and preventing asthma.1.3 HE dyeing demonstrated by Lung organization pathological section:After the antigen stimulated,the inflammatory of eosinophilic granulocyte and inflammatory cell of model group lung organization were increased obviously compared with that of the control group(P<0.01).2,Vitro method:This experiment discovered that the PCNA expression's average light density of blood serum including medicine was obviously higher than that of control group among three groups:normal control,high and low(P<0.05).It had non-significance difference compared with that of Dexamethasone group,and the same result was discovered by MTT method.The experiment proved:ASMC of big mouse was obviously restrained by blood serum group with medicine of high and normal group,and the result was the same as that of Dexamethasone group,but no obviously effect of low group.Maybe medicine density leaded to the result. The ASMC inhibit function had enhancement tendency along with the response time.Maybe explained that asthma was prevented and controlled through inhibition of His and proliferation of ASMC,and certain dosage and time dependence were included.Conclusions1,Vivo experimental:Suppressing cough function(symptoms improved and the content of IgE,EOS and EOS of BALF in blood were dropped. Bronchial tube and lung morphology) and mechanism function(the Eotaxin of chemotactic factor and ICAM-1 of adhesion molecule were controlled)were Confirmed for prescription of Patecy Lung and preventing asthma.2,Vitro experimental:For prescription of Patecy Lung and preventing asthma,Resistance asthma big mouse gas channel blocking function(Suppressed cell multiplication in the smooth muscle of bronchial tube) and mechanism(Suppressed smooth muscle cell's PCNA) were discovered.3,Freeing lung and relieving asthma and also removing heat-phlegm were defined to asthma.4,Experiment basis for the traditional Chinese compound prescription's development which relieving asthma was provided. |
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