Effects Of Liuweidihuang Drug-Containing Serum And Its Original Constituents Migratng To Blood On The Proliferation And Differentiation Of Rat Preadipocyte

[Objective] To confer the effects of Liuweidihuang drug—containing serum and its original constituents migrating to blood on the cAMP cGMP proliferation differentiation and the gene expression of peroxisome proliferator—activated receptor-γ2 (PPAR_γ2)of rat preadipocyte cells.[Methods] Firstly, the three original constituents migrating to blood were identified and determined by RP—HPLC. Secondly, Liuwei dihuang drug—containing serum and its three original constituents migrating to blood were added to the culture media of rat preadipocytes. The proliferation of cells, the expression of GPDH and lipid droplet accumulation in cytoplasm were observed and recorded. Thirdly, The changes of cAMP and cGMP contents were determined by using competitive protein binding assay, The last, the effects of Liuweidihuang drug—containing serum on the PPAR_γ2 gene and protein expression were assayed by semiquantitative RT-PCR[Results ]Loganin. paeoniflorin and paeonol were identified as the original constituents Liuweidihuang decoction migrating to blood. in the situation of yin-asthenia, the contents were more than normal. Compared with pills and decoction, they were the highest in micro-powder. Liuwei dihuang drug—containing serum and its three original constituents migrating to blood stimulated rat preadipocyte proliferation,inhibited GPDH up-regulation during differentiation,inhibited lipid accumulation in cell differentiation,decreased the amount of cAMP and restrained the expression of PPAR_γ2 mRNA in rat preadipocyte cells, loganin was one of the main constituents which decreased the amount of cAMP. micro-powder not only kept the effects of traditional decoction pieces, but also was better than pills and decoction.[Conclusion] Firstly ,the effects of Liuweidihuang prescription on the proliferation and differentiation of rat preadipocyte may supply especial drugs on molecule level and gene treatment of adipocyte illness. However the mechanism of it nourishing yin may through decreasing the amount of cAMP, affecting cAMP receptor, stimulating rat preadipocyte proliferation and inhibiting its' differentiation with Loganin. paeoniflorin , paeonol etc to improve the function of cells. Secondly, the absorption of active constituents were more than normal in yin-asthenia condition, it may create deep significance in clarifying the mechanism of Liuwei dihuang prescription affecting yin-asthenia and in researching the influence of different compound form of prepared drugs. Thirdly, the results of researching these three forms of prepared drugs above may bring valuable influence in controlling the quality and effects of Liuweidihuang prescription and researching the mechanism of micro-powder and traditionalChinese herbal medicine.