Research Of Acupuncture And Moxibustion On The Effects About Spleen Camp And CGMP Of CTX-induced Tumor-burdened Mice

Objective This topic using healthy male SPF KM mice to establishtumor-burdened mo del,then intraperitoneal injection of CTX150mg/kg,toestablish tumor-burdened i mmunosuppression model after che mothe rapy.Toobservation acupuncture and moxi bustion on cAM P and c GMP o fCTX-induced tumor-burdened mice.To explore the effects of acupunctureand mo xibustion on che mot herapy tumor-burdened i mmune system o fmice,reve al the acupuncture and moxibustion Improvin i mmunosuppressionafter chemotherapy,i mprove the mechanism of action of body’s immunefunction.Methods Select growth good source of S180sarcoma t u mors of mice,According to5×106tumor ce lls/0.2ml/only dose right axillary implantedtumor in mie,five days after can be in right armpit touch5-6mm siz esarcoma i n mice,ma rk the tumor-burdened mice model to cuccess.Accordingto the weight of tumor-burdened mice wer e randomly divided intotumor-burdened blank group,tumor-burened model group,tumor-burdenedacupuncture group,tumor-burdened moxibustion group.Tumor-burdenedblank group intraperitoneal injection0.9%saline in the sa me dose,theremaining three groups tumor-burdened mice by intraperitoneal injection of150mg/kg CTX,acc ording to phar macokinetic4h after mo del establishmentsuccess.In the same time period after successful mo del,tumor-burdenedacupuncture group,tumor burdened moxibustion group respectively take“dazhui”,”geshu”,”shenshu”,”zusanli”with acupuncture and mo xibustiontherapy,tumor-burdened blank group,tumor burden ed group accompaniedby fixed at the same ti me every day.After ac upuncture and moxibustiontreats three days,five days,killed respectively one batch of mice take out thecomplete spleen,homogenat e after weighing,use ELISA to detect thecontents of cAMP and cGMP in the spleen.Analyze the data using SPSS18.0statistical analysis software for Windows. Result1Treat me nt after3days， compared with the tumor-burdened model group，the spleen index of tumor-burdened acupuncture group increase, but therewas no significant difference (P＞0.05), the spleen index oftumor-burdened moxibustion group increase， there was significantdifference(P＜0.05); compared with tumor-burdened acupuncture group, thespleen index of tumor-burdened moxi bustion group inc rease, but therewas no significant difference (P＞0.05). Treat me nt after5days， comparedwith the tumor-burdened mo del group， the spleen index of tumor-burdenedacupuncture group and tumor-burdened moxibustion group increase, therewas significant difference(P＜0.05), compa red with tumor-burdenedacupuncture group, the spleen index of tumor-burdened mo xibustion groupdecline, but there was no significant difference (P＞0.05).2Treat me nt after3days， compared with the tumor-burdened model group，the content of c AMP in The spleen tissues of tumor-burdened acupuncturegroup and tumor-burdened mo xibustion group increase,there was significantdifference (P＜0.05), tumor-burdened acupuncture group is higher thantumor-burdened mo xibustion group,there was significant differenc e (P＜0.05);Treatment a fter5days,compared with the tumor-burdened modelgroup， the content of cAMP in The spleen t issues of tumor-burdenedacupuncture group and tumor-burdened moxibustion group increase, therewas signi ficant di fference (P＜0.05), tumor-burdened acupuncture groupis higher than tumor-burdened moxi bustion group,there was significantdifference (P＜0.05).3Treat me nt after3days， compared with the tumor-burdened model group，the content of c GMP in The spleen tissues of tumor-burdened acupuncturegroup and tumor-burdened mo xibustion group increase,there was significantdifference (P＜0.05), tumor-burdened acupuncture group is higher thantumor-burdened mo xibustion group,there was significant differenc e (P＜0.05);Treatment aft er5days， compar ed with t he tumor-burdened mo delgroup， the content of cGMP in The spleen t issues of tumor-burdenedacupuncture group and tumor-burdened moxibustion group increase, therewas significant difference (P＜0.05), tumor-burdened acupuncture group ishigher than tumor-burdened moxibustion group,there was significant difference (P＜0.05).4Treat me nt after3days， compared with the tumor-burdened model group，the ratio of cAMP/cGMP of tumor-burdened acupuncture group increase,there was significant difference (P＜0.05), the ratio of cAMP/cGMP oftumor-burdened mo xibustion group increase, but there was no significantdifference (P＞0.05). tumor-burdened acupuncture group compared withtumor-burdened mo xibustion group, there was no significant difference (P＞0.05).Treatment after5days，compared with the tumor-burdened modelgroup， the ratio of cAMP/cGMP of tumor-burdened acupuncture group andtumor-burdened moxibustion group increase,there was significantdifference (P＜0.05), tumor-burdened acupuncture group compared withtumor-burdened mo xibustion group, there was no significant difference (P＞0.05).Conclusion1Acupuncture and moxi bustion can improve the spleen index ofCTX-induced tumor-burdened mice, can reduce the da mage of i mmuneorgans spleen of CTX-induced tumor-burdened mice, improve immunefunction of CTX-induced tumo r-burdened mice.2CTX led to decline the content of c AMP in The spleen tissues oftumor-burdened mi ce, Acupuncture and moxibustion can improve thecontent of cAMP in The spleen tissues of tumor-burdened mice, thelymphocyte conversion rate as the cAMP increase rising, through improvethe content of cAMP can Inhibiting cell proliferation, make the cell DNAreplication、cell division、cell growth slowed; Promote cell differentiation,make DNA in the cells transcribe to InRNA, the synthesis of s pecificprotein further, increase the cell morphology, structure, biochemica lcomposition and function in the end, To i mprove the body’s i mmunefunction.3CTX led to decline the content of c GMP in The spleen tissues oftumor-burdened mi ce, Acupuncture and moxibustion can improve thecontent of cGMP in The spleen tissues of tumor-burdened mice, the contentof cGMP increasing fit the growth characteristic of cell proliferation, thecontent of cGMP increasing can Induce the increasing the content of DNA,Promote c ell mitosi s, inhibit cell differ entiation, Promote c ell proliferation guide regulate the body’s i mmune func tion.4CTX led to decline the cAMP/cGMP in The spleen tissues oftumor-burdened mi ce, Acupuncture and moxibustion can improve thecAMP/cGMP in The spleen tissues of tumor-burdened mice, increasing ofthe cAMP/cGMP, Enhance the body’s immune response ability, changing ofthe cAMP/cGMP is one of the body’s i mmune inhi bit reaction.