Model Of Stress-induced Depression In Rats In Chronic Liver Injury With The Establishment And Liver Stagnation Particle Intervention Role

Objective :Contrast with 40% CCL4 subcutaneous (12W), isolated and dependent chronic unpredictable mild stimulation (3W) model of the composite method of preparation of the combined stress of chronic liver injury animal model of depression, chronic liver injury to explore the combined stress-induced depression great Preparation of mouse model of the method. At the same time observe the effect of intervention Yanggan role Jieyu particles through the observation of the experimental rats liver function and weight, the observed changes in behavior, and electrochemical detection using high-performance liquid hypothalamic neurotransmitter levels were observed in liver, brain Sea pathological changes in the structure of horse, as well as parts of the brain hippocampus brain-derived neurotrophic factor nerve (BDNF), as well as hypothalamic arginine vasopressin (AVP) mRNA expression, Preliminary study Jieyu Yanggan particles combined treatment of chronic hepatitis mechanism of depression, further clarified the drug's liver, Jiangmei, the role of antidepressants and explain the molecular level from particles Yanggan Jieyu antidepressant mechanism of liver . Combined to study depression in chronic hepatitis lay the foundation for experimental research.Methods:1.Dividatur the rats into 8 groups randamly:blank group ,chronic hepatic injury model group (model group 1),chronic hepatic injury and stress depression model group (model group 2),Yigan Yiqi Jieyu Keli group ( control 1 group ),Fluoxetine Hydrochloride Capsules group (control 2 group ),the diminutive,midst,large dose of Yanggan Jieyu Keli(Treatment of diminutive,midst,large -dose group).2.The model was established by subcutaneous injection of carbon tetrachloride and separate bree with CUMS. The rats' weight,the Open-field test,sucrose intake, monoamine neurotransmitters in hypothalamus of rats were measured during the experiment,and liver pathology was observed.3. Measurement of body weight of rats in each group,dessert consumption, open-field test scores,liver function changes in hypothalamic neurotransmitters in the content, as well as liver and brain pathological changes in the hippocampus,the brain area CA1 hippocampus neurons BDNF positive cells and expression of hypothalamic AVP-mRNA expression. Results: Contrast with 40% CCL4 oil solution subcutaneous (12W), (1ml/kg, 2 times / week for 12 weeks), the merger raised alone and chronic unpredictable mild stimulation of the composite model can successfully merge the preparation of chronic liver injury in rat model of stress-induced depression; Study on the behavior of chronic liver injury with the impact of stress-induced depression in rats, Treatment of diminutive,midst-dose group can increase weight, improve the percentage of preference for dessert and open box test scores, and more effective than the control group 1 and 2 (P <0.05); Liver function of chronic stress-induced depression of liver injury in rats, the liver function of the target ,And comparison with control group1 and the control group 2 (P <0.01), Treatment of diminutive,midst-dose group that can reduce the elevated transaminases and jaundice, elevated to reduce the protein, And one more effective than the control group and control group 2. On the hypothalamic content of neurotransmitters, Treatment of diminutive,midst-dose group with the control group1, one better than the control group 1 (P <0.01), with the control group2, a considerable effect (P > 0.05); To improve the structure of the liver pathology, Treatment of diminutive,midst-dose group is better than the control group1 and control group 2 (P <0.01), be able to reduce liver cell necrosis; To improve the rat hippocampus of the brain structure,Treatment of diminutive,midst-dose group was significantly better than the control group 1 (P <0.01), compared with the control group 2, significant effect (P > 0.05); Hippocampus of the brain-positive neurons in BDNF expression, Treatment of diminutive,midst-dose group was significantly better than the control group 1 (P <0.05), compared with the control group 2, significant effect (P > 0.05); On the hypothalamic AVP-mRNA expression,Treatment of diminutive,midst dose group was superior to the control efficacy of one group (P <0.05), compared with the control group 2, diminutive,midst dose group a considerable effect (P > 0.05)。Conclusion:We can produce the chronic liver injury and separate bree with CUMS model of rats successfully by subcutaneous injection of carbon tetrachloride and separate bree with CUMS. 2. Yanggan Jieyu keli can adjust the weight, behavior, improve liver function, neurotransmitter content, reduce necrosis of liver cells, rat brain to improve the structure of hippocampus CA1 area and increase the BDNF-positive neurons in the expression of to reduce the hypothalamic AVP-mRNA expression. At its effect on the treatment of liver diseases of yigan Yiqi Jieyu keli more than particles in the treatment of depression with fluoxetine hydrochloride capsules considerable effect. In the treatment of depression with significant effects of fluoxetine hydrochloride capsules.3 Yanggan Jieyu Keli had the effect of liver protective and antidepressant, and is worthy of further study and development.