Studies On Secondary Metabolites Of Endophytic Fungi From Roots Of Erythrophloeum Fordii Oliv Studies On The Effective Chemical Substances Of Ligusticum Chuanxiong Hort. And Crataegus Pinnatifida Bge.

Part OneErythrophloeum fordii Oliv, Leguminosae, is one of the Chinese traditional herbal medicine.It widely distributed in some provinces in China, such as Guangxi, Guangdong, Fujian etc., and its bark and seeds are clinically used to facilitate blood circulation and dispersing blood stasis.Basing on our previous results of the studies on the cytotoxic compounds seperated from barks of Erythrophloeum fordii Oliv, we were going on the studies on secondary metabolites of endophytic fungi LYB-027from roots of Erythrophloeum fordii Oliv.. It has been identified that LYB-027is Aspergillus fumigatu.Chromatographic methods were applied to purify and separate its secondary metabolites.17compounds were isolated and their structures were identified by spectroscopic methods. They includes14diketopiperazine alkaloids,1oxaspiro lactam and2phenylacetamides.Compound1-6are new, and7-17are known. They are8R,9R-Dihydroxy-12R,18R-fumitremorgin C (1),8R-Methoxyl-9R-hydroxy-12S,18R-fumitremorgin C (2),8R-Methoxyl-9R,20-dihydroxy-12R18R-Fumitremorgin C (3),8R-Methoxyl-9R,19S,20-trihydroxy-12R,18S,19S-fumitremorgin C (4),8S,9S-Dihydroxy-spirotryprostatin A (5),6-Methoxyl-3-(3-methyl-2-butylene)-2-oxobrevianamide F (6), Brevianamide F (7), Fumitremorgin C (8),18-Oxotryprostatin A (9), N-Prenyl-cyclo-L-tryptophyl-L-proline (10), Tryprostatin B (11),8S,9R-Dihydroxy-12S,18S-fumitremorgin C (12), Cyclo-L-isoleucine-L-proline (13), Cyclo-L-leucine-L-proline (14), Pseurotin A (15),4-Hydroxy-phenylacetamide (16), N-acetyltyramine (17).Part TwoLigusticum chuanxiong Hort, the dried rhizome of Ligusticum chuanxiong (Umbelliferae) and Crataegus pinnatifida Bge., the dried ripe fruit of Crataegus pinnatifida are two major traditional Chinese medicines in China.Some rapid, convenient and efficient analytical methods, including HPLC-UV and HPLC-MS, has been used in the studies on the effective chemical substances in Ligusticum chuanxiong Hort. and Crataegus pinnatifida Bge.Tetramethylpyrazine(TMP), which is one of the main effective chemical ingredients contributing to the therapeutic effects of Ligusticum chuanxiong Hort., has always been employed as a chemical marker for quality control of it. Four methods were applied. First, abstracting TMP from processed chuanxiong respectively by using95%ethanol and water. Second, studying the same and different properties between TMP and TMP hydrochlorid by means of the preparation of TMP from TMP hydrochlorid. Third, separating TMP directly from processed chuanxiong by cold-soaking extraction and abstracting volatile oils using volatile oil extractor. Forth, repeating the experiment of the original literature in1977about the first isolation and identification of TMP from Ligusticum chuanxiong Hort. By comparing those experimental datas obtained from HPLC-UV and HPLC-MS, any evidence of the existence of TMP was found out. Therefore, it is doubtable that TMP is one of the main ingredients of Ligusticum chuanxiong Hort.. And then, the other major chemical ingredient ferulic acid was chosen as the chemical marker, and fully validated on precision, reproducibility and stability. The results demonstrated that it is available that ferulic acid was the bioactive chemical marker of quality control of chuanxiong.Crataegus pinnatifida Bge. mainly contains flavonoid compounds, which are the major bioactive ingredients. Hyperoside, one of flavonoid compounds, its precison, reproducibility and stability were fully validated. The results demonstrated that it is available that hyperosidewas the bioactive chemical marker of quality control of Crataegus pinnatifida Bge..