Establishment And Experimental Study Of Acute Mesenteric Ischemia Model In Rats And Diagnostic Value Of Ischemia Modified Albumin In Mesenteric Ischemia Patients

Background and Aims：The morbidity of acute mesenteric ischemia increased inrecent years. But it is difficult to diagnose in early stage and remains highmortality. Almost all basic research on acute mesenteric ischemia was carried outin the animal model with vascular ligated. The vascular ligated model can onlymimic extreme conditions of artery embolism. Pathological and physiologicalprocess of acute mesenteric venous thrombosis is different from ligated state varymuch. Differences between the pathophysiological process of clinical disease andthat of model lead to the deviation. Many papers about laser caused vascularstenosis or occlusion have been reported. We try to establish a new model of acutemesenteric ischemia by direct laser irradiation to mesenteric vascular and toobserve the change of serologic markers in the model.Materials and Methods: Different laser parameters were adopted to irradiateartery or vein in experimental rats after laparotomy and separate superiormesenteric artery and vein. Rats were observed24hours after irradiated.Compared with that of animals with the superior mesenteric artery or vein ligated,acute mesenteric ischemia model by laser irradiation induced could be confirmed.After that, sixty-six SD rats were randomly divided into11groups, each grouphad6rats. Group C0, C1and C2were control groups. These animals weresacrificed at immediately,1and2hours after laparotomy and separate superiormesenteric artery and vein, whose blood and intestinal samples were taken. Arterywas irradiated in group A1, A2and T1. Vein was irradiated in group V1, V2, P1, P2and T2. Blood and intestinal samples were taken1hour after irradiation ingroups A1and V1, and2hour after irradiation in groups A2, V2, T1and T2.Urokinase was administrated intravenous within15minutes in group T1and T2after irradiation. The samples of blood were taken in heart in all groups exceptgroup P1and P2, whose blood was taken from port vein.Results: Acute mesenteric ischemia model of rats can be established by laserirradiation with different laser parameters. Different severity of intestinal ischemiacan be achieved by varied laser irradiation parameters. Serum D-dimer in themodel tended to increase, but no statistically difference compared with the controlgroups. The levels of D-dimer in the portal vein blood was similar to systemcirculation, there was also no statistically difference. The content of intestinalfatty acid binding protein in the models increased at2hours after arteries or veinswere irradiated, significant difference could be found compared with the controlgroups. The content of ischemia modified albumin was no difference statisticallywithin the rat groups. But ischemia modified albumin test showed a100%positive rate in the patients (10cases). The elevations of ischemia modifiedalbumin in intestinal ischemia patients were higher than acute myocardialinfarction patients and had statistical difference.Conclusions: Acute mesenteric ischemia model of rats can be established bydirect laser irradiation. The levels of serum D-dimer and ischemia modifiedalbumin could not help to make a diagnosis of acute mesenteric ischemia in themodels. The levels of D-dimer in system circulation blood could represent thelevels in portal vein. The levels of intestinal fatty acid binding protein which weresignificantly elevated two hours after irradiation could help to make the diagnosisof acute mesenteric ischemia. No protective effect was found by urokinaseadministration intravenously in the models. Positive rate of ischemia modifiedalbumin test in clinical patients was100%.