The Study Between Human Papillomaviral Infection Typing Status Of Cervix In Different Nations In XIN Jiang And Treg Cells

The first chapter Epidemic research of human papillomavirus infection genotypes of cervical cancer in Urumqi in XinJiangObjective:To investigate the human papillomavirus infection genotypes and distribution in Urumqi Female Population reproductive tract.Methods:A questionnaire survey was conducted among the women aged18-64years in Urumqi by randomizing clustered sampling under stratification. All of patients received liquid-based cytology test(LCT) and HPV types. The patients who had abnormal cytological diagnosis were put up digital colposcopic exam. We Used pathologic diagnosis as a gold standard to diagnose CIN.Results:1.The positive rate of HPV was20.28%overall.2.The most prevalent genotypes of high-risk human papillomavirus (HR-HPV) were HPV16,58,52,18. The positive rate of HPV at different age groups, aged18-34,35-44,45-64years were18.07%,29.64%,7.64%, respectivly(P<0.05).3.The risk of cervical lesion significantly increased in women showing positive in cytology and HPV test. The HPV infection rate in different Occupations were different with the highest in service occupation(35.11%) while the lower appeared in works, white-collars and general residents. The HPV infection rate in works was significantly higher than that in general residents and white-collars. Conclusion:The most prevalent genotypes of high-risk human papillomavirus (HR-HPV) were HPV16,58,52,18in Urumqi. People aged35-44years are the most frequent infected groups in Urumqi. The HPV infection rate was highest among women having service related jobs and they should be closely monitored. The second chapter The investigation of reproductive tract human papillomavirus infection genotypes and distribution in Hans, Uygurs and Hazakhs of XinJiangObjective:To investigate the human papillomavirus infection genotypes and distribution in Hans, Uygurs and Hazakhs of XinJiang.Methods:A questionnaire survey was conducted among the women aged18-64years in Xinjiang by randomizing clustered sampling under stratification. All of patients received liquid-based cytology test(LCT) and HPV types. The patients who had abnormal cytological diagnosis were put up digital colposcopic exam. We Used pathologic diagnosis as a gold standard to diagnose CIN. Results:1. Among the total,the frequency of marriage, degree of culture, smoking history, the number of induced abortion, condoms using, the NO.of sexual partner, the NO.of sexual partner in recent five years and source of population had the relevant with human papilloma-virus infection of the Uygurs. Four variables(degree of culture,the NO.of sexual partner, condoms using and source of population)had significant in the multi-factor model.2.The positive rate of HPV of Uygurs was highest.,the comparison with Hans, Hazakhs had statistical significance.3.The most prevalent genotypes of high-risk human papillomavirus (HR-HPV) of Hans were HPV16,58,52,18,31. Uygurs’ were HPV16,58,31,52,68,39. Hazakhs’ were HPV16,18,58,33.4. The risk of cervical lesion significantly increased in women showing positive in cytology and HPV test. The comparison of incidence of C IN Ⅱ and C INⅢ of three nations had statistical significance. Uygurs’ were the highest.Conclusion:Degree of culture and condoms using were the protect factors, the NO.of sexual partner and source of population were the risk factors. The positive rate of HPV of Uygurs was highest.The genotypes of high-risk human papillomavirus (HR-HPV) of three nations were different. The risk of cervical lesion significantly increased in women showing positive in cytology and HPV test in three nations. The third chapter Research of mechanism of immune evasion in human papilloma viruse and CD4+CD25+CD127-Treg in cervical cancer of UygursObjective:To evaluate the expressions of CD4+CD25+CD127lo/-T cells in peripheral blood and TGF-β、IL-10and IFN-γ in serum from patients of Uygurs with cervical cancer. Through detect the level of CD4+CD25High T cell and the Foxp3in peripheral blood of cervical cancer patients of Uygurs,to investigate the effect of cervical cancer pathogenesis of CD4+CD25High T cell and the Foxp3of Uygurs. To investigate the correlation between human papilloma viruse(HPV) and CD4+CD25+CD127-T cells (Treg) of Uygurs.Methods:66patients with cervical cancer,35patients with CINII-Ⅲ,45patients with CINI and40normal controls were included in this study. The percentage of CD4+CD25+CD127lo/-T cells、CD4+CD25HighT population in CD4+T cells and T cells were evaluated by flow cytometric analysis. TGF-β、IL-10and IFN-γ were measured by ELISA.To compare the change of targets of preoperative and posto- perative of cervical cancer patients. The expression of Foxp3was evaluated by reverse transcription PCR. Typing of HPV (human papil-loma viruse) was evaluated by Nucleic acid amplification typing kit.Results:1.The percentage of CD4+CD25+CD127lo/-T cells of CD4+T lymphocytes in peripheral blood from patients with cervical cancer and CINⅡ-Ⅲ and contents of cytokine were significantly difference in comparison with that in patients with CINI and in normal controls (P<0.05).2.The CD4+CD25+CD127lo/-T cells percentage in cervical cancer and CIN patients was negatively correlated with IFN-γ (r=-0.585, P=0.000).The CD4+CD25+CD127lo/-T cells percentage in cervical cancer and CIN patients was positive correlated with TGF-β、 IL-10(r=0.690, P=0.000, r=0.827, P=0.000).3. The percentage of CD4+CD25+CD127lo/-T cells of CD4+T lymphocytes in peripheral blood from patients with cervical cancer and contents of cytokine of preoperative were significantly difference in comparison with that in postoperative (P<0.05).4.The expression of Foxp3in CD4+CD25+T cell from patients with cervical cancer were significantly difference in comparison with that in patients with CINI and in normal controls (P<0.05).5. The CD4+CD25HighT cells percentage in cervical cancer and CINⅡ-Ⅲ patients was positive correlated with Foxp3mRNA (r=0.853, P=0.000; r=0.586, P=0.000).6. Treg was positive correlated with HPV (r=0.560, P<0.05) Conclusion:CD4+CD25+CD1271o/-Treg, TGF-β,IL-10and IFN-y participate the process of immune evasion of cervical cancer of Uygurs. HPV was intimate correlated with Treg in cervical cancer of Uygurs.