Effector Mechanisms Of Shenqi Compound Recipe In Treating Diabetic Microangiopathy

Objective: To explore the intervention mechanism and observe the effect of Shenqi Compound Recipe (SCR) in treating Goto-Kakizaki (GK) rat model of diabetes with microvascular lesions in kidney and retina, on the theortic basis of six-channel pattern identification for dispersion-thirst (xiao ke), to identify the synthesis effect of SCR in treating diabetic microangiopathy (DM), and finally, bring experimental evidence for SCR in the treatment of DM.Methods:50GK rat models with6-month disease duration were used and randomly pocessed into5groups:negative control group, SCR group, ramipril group, calcium dobesilate group and metformin group, with10rats in each group and another10normal SD (Sprague Dawley) as blank control. All the rats were intragastrically administered with SCR and control drigs for12weeks. Renal morphological observation was performed using light microscopy. Renal function was detected using oxidase test. The expression of nuclear factor kappa B (NF-κB) was detected using immunohistochemical staining method. Flash electroretinogram was used to evaluate the retinal function. Periodic acid-schiff staining was used to detect retinal capillary vascular basement membrane thickness. And sciatic nerve electrophysiology was detect to evaluate the peripheral nerve conduction velocity.Results:SCR was indicated to improve the renal function, reduce the pathologic changes in kidney, decrease the expression of NF-κB in GK rats, thus protect GK rats from renal damages. Experiments showed that SCR played a role in rehabilitating the amplitude both of a and b waves in flash electroretinogram, and shortening the peak latency both of a and b waves, thus improving the damaged visual function and reducing retinal capillary vascular basement membrane thickness. SCR was also indicated to restore both sensory nerve conduction velocity as well as motor nerve conduction velocity of sciatic nerve, and shorten the sensor-reaction latent period, protect diabetic GK rats from peripheral nerve injury.Conclusion:SCR can significantly improve the renal function, visual function and peripheral nerve function of diabetic GK rats via multiple treatment targets and multiple ways, to reduce the pathological damages both in kidney and retina, thus playing a comprehensive role in the treatment of DM.