Effect Of Qi Phlegm Promoting Blood Circulation On AS Adhesion Molecules And With Dyslipidemia Syndrome Correlation

Objective:To study the effect of "Tiaozhitongmai" granules on serum lipid,atherosclerosis plaque and NF-κ B,ICAM-1and LFA-1’s expressions in the aortic arch of ApoE-/-Mice. Provided experimental foundation for the method of reinforcing Qi as well as removing phlegm and stagnant blood to treat blood lipid abnormality and AS. To study the distribution rule of TCM syndromes of dysl ipidemia, and the relationship between that and serum level of TC, TG, HDL-C, sICAM-1and sVCAM-1.Methods:In the part of animal research, Six week-old male C57BL/6mice and ApoE-/-mice were divided into control group, model group, Xuezhikang group and "Tiaozhitongmai" granule group (low-,medium-, high-dosage) There were8mice in each group. They were fed by chow diet combined with medicine for12weeks. Changes in serum lipid, sICAM-1and sVCAM-1, as well as that of atherosclerosis and expressions of NF-k B, ICAM-1, and LFA-1in aortic arch were monitored by automatic biochemical serum analysis machine, ELISA, HE staining, and Immunohistochemical staining respectively. In the part of clinical research, we selected120patients with dyslipidemia according inclusion and exclusion criteria. Collecting their general information and finishing clinical research form after signed informed consent form. Then use automatic biochemical serum analysis machine and ELISA to monitor patients’serum level of lipid, sICAM-1and sVCAM-1respectively. Use statistic approach to analyze the relationship between patients’medical indexes of a test and TCM syndromes.Results:1By comparison to the control group, the serum levels of TC, LDL-C, and HDL-C have significantly increased in ApoE-/-mice (P<0.05), so was the serum levels of TG in the modeling group and in "Tiaozhitongmai" granule low-dosage group (P<0.05).2Compared with control group, serum level of sICAM-1and sVCAM-1significantly increased in ApoE-/-mice(P<0.05), and compared with model group, serum level of sICAM-1and sVCAM-1significantly decreased (P <0.05)3The HE staining showed that there were no atherosclerosis plaques formed in control group, yet vulnerable atherosclerosis plaques was formed in model group, and that stable atherosclerosis plaques were also formed in "Xuezhikang" group and "Tiaozhitongmai" granule high-dosage group. 4In comparison to the control group, NF-κ B、ICAM-1and LFA-1expression significantly increased in ApoE-/-mice (P<0.05), while, expressions of NF-κ B、ICAM-1and LFA-1decreased (P<0.05) in "Tiaozhitongmai" granule high-dosage group comparing to the modeling group.5In clinical research, we found the distribution rule of TCM syndrome was:Ganshenyinxu (40.83%)> Yinxuyangkang (25.83%)> Tanzhuozuzhi (20.83%)>Pishenyangxu (8.33%)> Qizhixueyu (4.17%)6The syndrome type of TC level from high to low in the order was: Qizixueyu> Ganshenyinxu> Tanzhuozuzhi> Yinxuyangkang> Pishenyangxu>normal group. Syndrom of Ganshenyinxu, Tanzhuozuzhi, Yinxuyangkang and Qizhixueyu had higher TC level than normal group. Syndorm of Qizhixueyu had higher TC level than that of Ganshenyinxu and Yinxuyangkang.7The syndrome type of TG level from high to low in the order was: Tanzhuozuzhi> Yinxuyangkang> Ganshenyinxu> Pishenyangxu> Qizhixueyu>normal group. Syndrom of Tanzhuozuzhi and Yinxuyangkang had higher TG level than normal group. Syndorm of Tanzhuozuzhi had higher TG level than that of Yinxuyangkang.8The syndrome type of HDL-C level from low to high in the order was: Ganshenyinx<normal group<Yinxuyangkang<Pishenyangxu<Tanzhuozuzhi <Qizhixueyu. There was no significant difference between six groups.9The patients with dyslipidemia had higher serum sICAM-1level than normal group. The syndrome type of sICAM-1level from high to low in the order was:Yinxuyangkang> Ganshenyinxu> Pishenyangxu> Tanzhuozuzhi> Qizhixueyu> normal group. There was no significant difference between six groups.10The patients with dyslipidemia had higher serum sVCAM-1level than normal group. The syndrome type of sVCAM-1level from high to low in the order was:Ganshenyinxu> Tanzhuozuzhi> Yinxuyangkang> Pishenyangxu> Qizhixueyu> normal group. There was no significant difference between six groups.Conclusion:1"Tiaozhitongmai" granule appeared to be effective in decreasing the serum level of TG, sICAM-1and sVCAM-1, as well as the expressions of NF-κ B, ICAM-1, and LFA-1in the aortic arch of ApoE-/-mice.2The syndrome of Ganshenyinxu, Yinxuyangkang and Tanzhuozuzhi is one of the important syndrome types in patients with dyslipidemia, Pishenyangxu appear less, the lowest proportion is Qizhixueyu.3Dyslipidemia in patients with higher TC is closely related to the syndrome of Qizhixueyu, elevated TG and the syndrome of Tanzhuozuzhi connected, may identify those two syndrome based on serum TC and TG level.4The serum sICAM-1and sVCAM-1level of patients with dyslipidemia were significantly higher than normal people, that means the inflammatory reaction connected with AS had already happened in the early stage of dyslipidemia. But there was no correlation between serum adhesion molecular level and different syndrome types.