Study On The Recognition Of Azo Dye And Protein Moleculer By Molecularly Imprinted Technique

Molecularly imprinted technique (MIT) is a new method that contains tailor-made recognition sides for certain molecules. It is with many characteristics such as pre-determinative, specific and practical abilities of molecule recognition. It has been extensively applied in biomimics, purification, separation and enrichment fields.In this dissertation, MIT, mainly including the protein template imprinted technique, was studied. Four chapters are presented in the thesis.In chapter 1, in this review section, the principles of MIT, the preparation methods and the application of molecularly imprinted polymers (MIPs) were summarized. The current problems occurred in MIT and its development were analyzed and prospected.In chapter 2, a molecular imprinted polymer, 1-(2-pyridylazo)-2-naphthol (PAN), was synthesized withα-acrylic acid (MAA) as a functional monomer in the chloroform solvent by classical molecular imprinted technique. The polymer was characterized by IR and UV spectra and the equilibrium binding experiment. The adsorptive capability of the polymer was investigated in the study.In chapter 3, MIPs were prepared with albumin bovine serum (BSA) as template molecule, acrylamide as functional monomer and N,N'-methylenebisacrylamide as cross-linker by surface MIT in the presence of 0.45μm polyethylene filter membrane, and the selective permeation was studied for the BSA and its analogues hemoglobin bovine blood (HBb). A higher permeation selectivity to BSA was obtained when the condition was selected as polymerized concentration of template molecule, functional monomer and cross-linker was 0.004,0.096,0.020 g/mL, respectively.In chapter 4, a new BSA protein molecular imprinted nanotubes (PMIN) was synthesized by a surface MIT on the surface of carbon nanotubes. The selective recognition of PMIN was evaluated by adsorption experiments. Comparing the non-imprinted nanotubes (nPMIN), the results showed that the PMIN exhibited excellent recognition and selective ability to BSA due to large surface area of the nanotubes and the protein imprinted sites be located at the surface results in large protein molecule binding capacity of the PMIN. Moreover, the components of PMIN were analyzed by IR and its configurations were observed by SEM.