Molecularly Imprinted Biosensor Based On Nickel-polyacrylamide Sensitive Membrane

Dopamine(DA)is a very important neurotransmitter in human body,its role is mainly in the control of metabolism,cardiovascular,renal system,and so on.The abnormal level of DA in human body is related to many common neurological disorder.Adenine(Ade)is one of the four bases that make up DNA and RNA molecules.It not only participates in the synthesis of DNA and RNA in the body,maintaining the metabolic function of the organism,but also promotes the proliferation of white blood cells in the absence of white blood cells.Therefore,the detection of the levels of dopamine and Adenine in the complex physiological environment is crucial.Molecularly imprinted polymer(MIP)films prepared in Situ have the following advantages:they can retain their original shape,adhere firmly to the surface of basis,and structure can be controlled.In this paper,a molecular imprinting biosensor based on nickel(Ni)-Polyacrylamide sensitive membrane is designed,which is based on electropolymerization of AM monomer in the presence of Ni and template molecules,ni-PAM-MIP Matrix was prepared on a glassy carbon electrode(GCE)by in-situ preparation method.Ni²⁺was used as oxidant to promote the polymerization of AM monomer and as Filler to prepare three-dimensional porous PAM-MIP Matrix.Based on the Ni-PAM-MIP Sensor,the dual response of Dopamine(DA)and Adenine(Ade)can be demonstrated in the PH range of 5.0?9.0.The linear concentration range varies with Ph.the concentration ranges of DA and Ade are 0.6?200?A/?M and 0.4?300?A/?M,respectively.The detection limit of DA(s/n=3)is 0.12?0.37?M,and Ade is0.15?0.36?M.In addition,due to its excellent anti-interference ability and long-term stability,Dual-MIP sensor has high reliability in detecting DA and Ade in human serum.Therefore,this work is expected to provide new strategies for the construction of multi-target response electrochemical biosensors and the reliable determination of small molecules with high selectivity and stability.