| In this study, a vivparous lizard Eremias multiocellata which were captured in Tianzhu, Gansu province was used for the study. To investigate the effect of different incubation temperature on the offspring sex, morphological phenotypes and motion ability.We took the combination of laboratory and field experimental methods, adult lizards were kept in four different constant temperatures (25±0.5℃,29±0.5℃,31±0.5℃,35±0.5℃), and established a semi-natural condition fence as the control experiment. Pregnant female lizards' body temperatures were detected during the whole breeding process, and the start of temperature control was considered as the developmental zero of pregnant females. After born from different incubation conditions, the morphological phenotypes of offspring were measured, as well as athletic ability.The results showed that under different incubation temperature, the pregnant female lizard's body temperature raised with incubation temperature, body temperature of pregnant female lizards in different temperature groups were significant different. Embryo incubation time shortened with increased incubation temperature, and the relationship between incubation temperature and incubation duration was nonlinear. Eremias multiocellata offspring sex significantly affected by different incubation temperatures, with the incubation temperature raised, the proportion of offspring of males increased from 40.9% when 25℃to 66.67% when 35℃. Different incubation temperatures had a significant effect on morphological phenotypes, including tail length, head length, head width, forelimb length, hind limb length and eye length, but there is no significant impact on offspring's body weight, SVL and tympanic membrane length. These results indicate that different incubation temperatures could significantly affect the pregnant female lizards' body temperature and then impact on the whole embryo during development.
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