Extraction Separation And Determination Of Useful Ingredients From Indigowoad Root

In allusion to farming products' safety problem , this thesis analysed the present actuality in the exploitation of plant feedingstuff additive and the advantage of our country in officinal plants, the methods of effective ingredient extraction, and also elucidated the resource predominance, characteristics, pharmacological function and main chemic ingredient of indigowoad root.Based on the analysis and comparison of various methods of mensuration of effective ingredients of indigowoad root,phenol-vitriolic colorimetry has been choosen to be the method of mensuration of amylose in indigowoad root, and standard amylose graph has been made,in which c=22.476A+0.5309, correlation rate r=0.9970; Direct colorimetry has been choosen to be the method of mensuration of indigotin and Indirubin,and based on all above,the maximal absorb wavelenghes of indigotin and Indirubin have been determined through experiment.As to Hebei indigowoad root, hot water extraction and microwave extraction methods have been used to extract polysaccharides of indigowoad root.The following conclusion has been reached through experiment on the ethanol concentration and the times of ethanol precipitation:in ethanol precipitation,if the ethanol concentration is 80%, the production rate of polysaccharides of indigowoad root reaches the topmost of 13.38%;When the times of ethanol precipitation is increased,although the content of polysaccharides increased distinctly (reached 80.253%), production rate reduced(14.83%),and, a great deal of organic solvent must be used in secondary ethanol precipitation,making the cost exorbitant.In this thesis, ethanol precipitation (microwave extraction menthod) has been used for the first time to extract polysaccharides of indigowoad root ,with the dunking time, microwave power and microwave processing time deeply studied respectively,and the optimal craftwork worked out through (positive and negative)experiments,and combined for the first time with traditional hot water extraction method to increase the production rate and content of polysaccharides 12.432% and 5.847% respectively higher than that of traditional method, acquired satisfactory effects.Great difference in production rate and contents of polysaccharides of indigowoad root from different places has been determined by experiments on indigowoad root from different places(Hebei,Inner Mongolia,Shanxi and Henan,etc.).The best parameters of technology have been determined through single factor and positive cross test on indigotin and Indirubin respectively: medicinal materials granularity is 40 eye,extractiontime is 4hrs, ethanol concentration is 75%.