The Pure Microbial Species Ethanologenbacterium Sp. Nov R3 For Anaerobic Bio-Hydrogen Production Of Fermentation

The significant research results are achieved through anaerobic and fermentative bio-hydrogen production in batch reactor and CSTR of 18.8L where plant straw from agricultural waste and molasses wastewater are the materials for hydrogen production and pure microbial species EETHANOLOGENBACTERIUM SP.NOV R3 are hydrogen-producing bacterium. The result is as follow:Batch culture is used to study the hydrogen-producing bacterium Eethanologenbacterium sp.nov R3 for its hydrogen production capacity and regulation method.The results show that the quantity of hydrogen production of Eethanologenbacterium sp.nov R3 R3 is maximum at glucose concentrations to 12g/L;different substrates have much influence on hydrogen production capacity of the bacterium Eethanologenbacterium sp.nov R3 and that capacity with hexose and disaccharide as substrate is higher than that with pentose;yeast extract as organic nitrogen source is the most effective nutrient for the quantity of hydrogen production of Eethanologenbacterium sp.nov R3,but it is taken into account comprehensively that beef extract,peptone,yeast extract as organic nitrogen source is the best choice;with the bacterium Eethanologenbacterium sp.nov R3 preferring neutral temperature,it is between 30～35℃;the best pH is at about 5.5.Plant straw as material mashes up into serofluid and then after high temperature sterilization on it,the degradation experiment of different time and temperature is made by acetate and hydrochloric acid to obtain the saccharification liquid and hydrogen is produced in air bath oscillator.The result tells us that the temperature 40℃is the best one for degrading the straw with acetate while 20℃is the best for that with hydrochloric acid;the optimal time is both 1h for degrading straw by use of acetate and hydrochloric acid;less quantity of hydrogen is in condition of the saccharification liquid degraded into through weak acids like acetate compared with through strong acids like hydrochloric acid.The starting and operation of system of fermentative bio-hydrogen is studied by CSTR as reaction equipment of anaerobic and fermentative bio-hydrogen and pure microbial species Eethanologenbacterium sp.nov R3 as strains of hydrogen production.The results showed that hydrogen production capacity can be continuous in the CSTR in conditions of fermentative substrate of molasses wastewater;in the CSTR the temperature is between 35±1℃and HRT is 8h;16 days are spent on the stability of hydrogen production and 6.6L/d of biogas production and 59.4%of hydrogen content are both based on the average removal rate 28%of COD;the situation of ethanol and acetate as main fermentative terminal products,which account for 90% of total amount,belong to that of ethanol-type fermentation;pH of the final effluent changes between 4.0～4.5 and the addition of Sodium Hydroxide into CSTR can make the effective adjustment of pH.From the study,the strains Eethanologenbacterium sp.nov R3 of high efficient hydrogen production can be applied to bio-hydrogen process of continuous flow.