Using Fruit Processing Waste As Substrate For Anaerobic Bio-Hydrogen Production Of Fermentation

Through partial training and continuous flow training, pure strain R3 is used in this study to produce hydrogen. The subtrates are the wates produced by fruit processing industries. The purpose of this study is not only the production of hrdrogen but also the waste degration. In partial cultivation experiment, the apple and orange industrial waste residue are used as substrates. In the continuous flow training experiments, the substrate is grape drink wastewater. The ones discussed in this study are the best process control parameters of R3 in different substrates and different cultivation types.In partial cultivation experiment, using apple waste residue, when its dosage, initial pH value and reaction system are respectively 400g/L,6.0 and 30℃～35℃, the reaction system achieves the best state. In these conditions, hydrogen production yield and hydrogen content are respectively 423.51ml/L and 48.9%; Cell concentration achieves 0.224g/L; Average rate of hydrogen production is 2.021mmolH2/gCDW·h; The contents of ethanol and acetic acid are respectively 1896.51mg/L and 1032.88mg/L; Fermented eventually pH value is 3.33. Using orange waste residue, when its dosage, initial pH value and reaction system temperature are respectively 100g/L,6.5 and 25℃～35℃, the reaction system achieves the best state. In these conditions, hydrogen production yield and hydrogen content are respectively 540.94mL/L and 50.5%; Cell concentration achieve 0.27g/L; Arerage rate of hydrogen production is 1.988 mmol H2/g CDW.h; The contents of ethanol and acetic acid are respectively 1901.8mg/L and 1200.27mg/L; Fermented eventually pH value is 3.13. These conclusions show that:using orange waste residue as substrate, the hydrogen production effectiveness is better. The carbohydrates in oranges are much easier to use by R3 for producing hydrogen. But the substances in oranges inhiting the cells of R3 growth is more than that in apples. Once the orange consumption is higher,the cell concentration and hydrogen production yield dropped deeply.Using grape drink wastewater as substrate, In CSTR, R3 is used to produe Hydrogen. When the reactor is in a stable operation stage, biogas production yield and hydrogen content can achieve 4.66L/d and 58.96%; COD removal rate is about 26%; Fermentation type is ethanol type fermentation effluent pH value is 3.5.In the continunous flow experiments, influent pH value is adjusted. When influent pH value is 5.5, the capacity of R3 producing hydrogen is best. At this time, biogas production yield and hydrogen content are respectively 6.85～8.86L/d and 39.44%～65.13%; COD removal rate is still 26%; Effluent pH value is 4.38 which is suitable niche of R3. In total volatile acid, the concentration of ethanol and acetic acid are respectively 706mg/L and 446mg/L. R3 always keep the characteristics of ethanol type fermentation.