Study On The Immobilization And Application Of White-Rot FungI In Diazodinitrophenel Wastewater Treatment

In this paper, self-breeding culture of white rot fungi WX and explosivediazodinitrophenel (DDNP) wastewater as the research object, studied the white rot fungusculture, domestication and immobilized, and treatment the pretreatment DDNP wastewater bythe Fe-C micro-electrolysis using of immobilized white-rot fungus, focusing on theimmobilized of white-rot fungi WX and the treatment conditions and degradation of theDDNP wastewater by the immobilized white-rot fungi WX.Breeding out, cultivated and domesticated of white rot fungus WX which could beefficiently degrade DDNP wastewater, determined the growth cycle of W.X fungi by theexperiment; Immobilized experiments confirmed the optimal conditions of sodium alginateembedding method and polyvinyl alcohol (PVA) crosslinking method immobilized white-rotfungus:(1)The optimal conditions of sodium alginate embedding method:3% sodium alginate, 0.3% diatomite and 3% fungi mixed with each other, then trickledonto the 3% CaCl₂ solution with constant flow pump(the flow rate was12mL·min^-1) , naturalstate of pH value.(2)The optimal conditions of polyvinyl alcohol (PVA) crosslinking method:8% polyvinyl alcohol and 3% fungi mixed with each other, then trickled onto thesaturated boric acid solution contained 2% CaCl₂ with constant flow pump (the flow rate was8mL·min^-1), adjusting the pH value of crosslinker to 6.7 using of Na₂CO₃, the additives ofPVA contained 0.8% sodium alginate, 0.8% active carbon powder, 0.3%CaCO₃,2%SiO₂.The experiment conditions of treatment the pretreatment DDNP wastewater by the Fe-Cmicro-electrolysis using of immobilized white-rot fungus were: influent concentration (COD)≤800mg·L^-1, pH = 5, immobilized globule dosage 200g·L^-1, RT = 5d, 30℃constanttemperature oscillation shaker (the revolution of shaking table was 140rpm) treatment; the quality of the effect on DDNP treatment by the five immobilization method was: activatedcarbon immobilized>PVA immobilized>peanut shell immobilized>sodium alginateimmobilization>sawdust immobilized; After five days, COD removal rate reached 83.7%, thedecolorization rate was 92.5%.After five steps of treatment, which were neutralization and explosion elimination,hydrolytic acidification, Fe-C micro-electrolysis, regulating the influent concentration ofbiochemical treatment (diluted five times), and treatment by immobilized W.X fungi, the colorof DDNP wastewater down to 100 times from 62500 times, decolorization rate was 99.9%,COD down to 129.9mg·L^-1～180.9mg·L^-1 from 29481.6m·L^-1 of the raw water, CODremoval rate could be reach 99%. pH value was about 6, effluent quality basically meet theDDNP wastewater discharge standards for water pollutants from ordnance industry initiatingexplosive material and relative composition(GB14470.2—2002).The research provided the basis of the application for biochemical treatment DDNPwastewater and other explosive wastewater, and it also provide a reference for the applicationof immobilized white-rot fungi technology in explosives and other high-concentration andhardly degradable organic wastewater.